Production of Lactosucrose from Sucrose and Lactose by a Levansucrase from Zymomonas mobilis
- Production of Lactosucrose from Sucrose and Lactose by a Levansucrase from Zymomonas mobilis
- Han, Woo-Cheul; Sun-Ho Byun; Mi-Hyun Kim; Eun Hwa Sohn; Jung Dae Lim; 엄병헌; Chul Ho Kim; Soon Ah Kang; Ki-Hyo Jang
- Glucose oxidase; lactosucrose; levansucrase; Zymomonas mobilis
- Issue Date
- Journal of microbiology and biotechnology
- VOL 19, NO 10, 1153-1160
- Lactosucrose (4G-β-D-galactosylsucrose) is an oligosaccharide
consisting of galactose, glucose, and fructose. In this study,
we prepared lactosucrose from lactose and sucrose using a
levansucrase derived from Zymomonas mobilis. Optimum
conditions for lactosucrose formation were 23oC, pH 7.0,
18.0% (w/v) lactose monohydrate, and 18% (w/v) sucrose
as substrates, and 1 unit of enzyme/ml of reaction mixture.
Under these conditions, the lactosucrose conversion efficiency
was 28.5%. The product was purified and confirmed to be
(1→2)-β-D-fructofuranoside, or lactosucrose. A mixed-enzyme
system containing a levansucrase and a glucose oxidase
was applied in order to increase the efficiency of lactose
and sucrose conversion to lactosucrose, which rose to 43.2%
as a result.
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