Production of Lactosucrose from Sucrose and Lactose by a Levansucrase from Zymomonas mobilis

Title
Production of Lactosucrose from Sucrose and Lactose by a Levansucrase from Zymomonas mobilis
Authors
Han, Woo-CheulSun-Ho ByunMi-Hyun KimEun Hwa SohnJung Dae Lim엄병헌Chul Ho KimSoon Ah KangKi-Hyo Jang
Keywords
Glucose oxidase; lactosucrose; levansucrase; Zymomonas mobilis
Issue Date
2009-10
Publisher
Journal of microbiology and biotechnology
Citation
VOL 19, NO 10, 1153-1160
Abstract
Lactosucrose (4G-β-D-galactosylsucrose) is an oligosaccharide consisting of galactose, glucose, and fructose. In this study, we prepared lactosucrose from lactose and sucrose using a levansucrase derived from Zymomonas mobilis. Optimum conditions for lactosucrose formation were 23oC, pH 7.0, 18.0% (w/v) lactose monohydrate, and 18% (w/v) sucrose as substrates, and 1 unit of enzyme/ml of reaction mixture. Under these conditions, the lactosucrose conversion efficiency was 28.5%. The product was purified and confirmed to be O-β-D-galactopyranosyl-(1→4)-O-β-D-glucopyranosyl- (1→2)-β-D-fructofuranoside, or lactosucrose. A mixed-enzyme system containing a levansucrase and a glucose oxidase was applied in order to increase the efficiency of lactose and sucrose conversion to lactosucrose, which rose to 43.2% as a result.
URI
http://pubs.kist.re.kr/handle/201004/36393
ISSN
1017-7825
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KIST Publication > Article
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