Qualitative and quantitative analysis of microbial community structure in the sequencing batch reactor for enriching ANAMMOX consortium

Title
Qualitative and quantitative analysis of microbial community structure in the sequencing batch reactor for enriching ANAMMOX consortium
Authors
배효관정진영
Keywords
혐기성 암모늄 산화; 농후배양; 계통분류학적 분지도; real-time quantitative PCR; Anaerobic Ammonium Oxidation; Enrichment; Phylogenetic Tree
Issue Date
2009-10
Publisher
大韓環境工學會誌; Journal of Korean Society of Environmental Engineers
Citation
VOL 31, NO 10, 919-926
Abstract
Enrichment of anaerobic ammonium oxidation (ANAMMOX) bacteria is the essential step for operating full-scale ANAMMOX bioreactor because adding a significant amount of seeding sludge is required to stabilize the ANAMMOX reactor. In this study, the enrichment of ANAMMOX bacteria from an activated sludge using sequencing batch reactor was conducted and verified by analyzing changes in the microbial community structure. ANAMMOX bacteria were successfully enriched for 70 days and the substrate removal efficiencies showed 98.5% and 90.7% for NH4 + and NO2 - in the activity test, respectively. The phylogenetic trees of Planctomycetes phylum showed that the diverse microbial community structure of an activated sludge was remarkably simplified after the enrichment. All 36 clones, obtained after the enrichment, were affiliated with ANAMMOX bacteria of Candidatus Brocadia (36%) and Candidatus Anammoxoglobus (64%) genera. The quantification using real-time quantitative PCR (RTQ-PCR) revealed that the 16S rDNA concentration of ANAMMOX bacteria was 74.8% compared to the granular ANAMMOX sludge obtained from an upflow ANAMMOX sludge bed reactor which had been operated for more than one year. The results of molecular analysis supported that the enriched sludge could be used as a seeding sludge for a full-scale ANAMMOX bioreactor.
URI
http://pubs.kist.re.kr/handle/201004/36444
ISSN
1225-5025
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KIST Publication > Article
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