Terminal-Restriction Fragment Length Polymorphisms (T-RFLP) Analysis for Microbial Community in Methane Gas Production Process

Title
Terminal-Restriction Fragment Length Polymorphisms (T-RFLP) Analysis for Microbial Community in Methane Gas Production Process
Authors
배재상김세일주동훈성현제박성범엄영순상병인
Keywords
T-RFLP; Archaea; PCR; Microbial community; Anaerobic digester
Issue Date
2009-10
Publisher
한국생물공학회
Abstract
Anaerobic digesters for the methane gas production from food wastes were comprised with methane fermentation and stabilized reactor. During one years, 25 Samples were collected from the reactors and the microbial communities were analyzed by T-RFLP using two different primer sets (63F-FAM/1389R and 27F-FAM/1492R) and three restriction enzymes (AluI, RsaI, and HhaI). Among these combinations, the combination of 63F-FAM/1389R primer set and restriction enzyme AluI showed the highest discrimination level of the microbial community and T-RFLP analyses with this combination could be used as a method for addressing microbial diversity of the anaerobic digesters.
URI
http://pubs.kist.re.kr/handle/201004/36508
Appears in Collections:
KIST Publication > Conference Paper
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