The bacterial community of waste treatment bioreactor

The bacterial community of waste treatment bioreactor
tidal flat; pyrosequencing; 16S rRNA; community
Issue Date
2009 International meeting of the federation of Korean Microbiological Societies
The bacterial community of the waste treatment bioreactor was analyzed using pyrosequencing (GS-FLX titanium). A total of 45,679 sequence reads with valid barcode were obtained from four samples. The number of the sequence reads was reduced to 35,844 after processing. The average length of the processed sequence reads was 462 bases. Each sequence read was assigned to taxonomic group using Eztaxon-extended database, holding manually annotated 16S rRNA gene sequence information of both cultured type strains and uncultured representatives. The majority taxa of the community were represented by three phylum; Chloroflexi, Synergistetes, and Firmicutes. Anaerolinaceae, Aminobacterium, and Clostridiales were accounted for the majority of Chloroflexi, Synergistetes, and Firmicutes respectively. The closest relatives of the most sequence reads were uncultured phylotypes. Approximately, only 60% of total sequence could be assigned to genus. It indicated that the community of the reactor was consisted with many novel phylotypes which were not previously reported. In conclusion, the bacterial community of the waste treatment bioreactor was simple in phylum level, but most of the sequences reads were close to previously uncultured phylotpyes and only half of sequence reads could be assigned to genus.
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