Isolation and characterization of hexanoic acid producing megasphaera sp. nov. BS-4 from cow rumen
- Isolation and characterization of hexanoic acid producing megasphaera sp. nov. BS-4 from cow rumen
- 전병승; 김세일; 최기은; 엄영순; 상병인
- Hexanoic acid; Megasphaera; Cow rumen
- Issue Date
- , 142-142
- The hexanoic acid could be converted to valuable chemicals, for example, adipic acid (nylon’s material) by
oxidation process and hexanol by hydrogenation. For the isolation of hexanoic acid producing bacteria from
cow rumen, RCM (Reinforced clostrdia medium) with glucose as carbon source and hexanoic acid as selection
pressure was used. Isolated bacteria was designated Megasphaera sp. nov. BS-4 and its 16s rDNA showed 94 %
similarity with Megasphaera paucivorans DSM 16981. Physiological test is performed by API kit and API 50CH
showed that Megasphaera sp. nov. BS-4 uses D, L-arabinose, inositol and fructose. Especially, the isolated
bacterium showed the maximum hexanoic acid production, around 4g/L without pH adjustment, when fructose
was used as a carbon source.
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