Production of Cellulosic Ethanol in Saccharomyces cerevisiae Heterologous Expressing Clostridium thermocellum Endoglucanase and Saccharomycopsis fibuligera β-glucosidase Genes
- Production of Cellulosic Ethanol in Saccharomyces cerevisiae Heterologous Expressing Clostridium thermocellum Endoglucanase and Saccharomycopsis fibuligera β-glucosidase Genes
- Eugene Jeon; Jeong-eun Hyeon; 서동진; 서영웅; 김승욱; 송광호; 한성옥
- Issue Date
- Molecules and cells
- VOL 28, 369-373
- Heterologous secretory expression of endoglucanase E
(Clostridium thermocellum) and β-glucosidase 1 (Saccharomycopsis
fibuligera) was achieved in Saccharomyces
cerevisiae fermentation cultures as an α-mating factor
signal peptide fusion, based on the native enzyme coding
sequence. Ethanol production depends on simultaneous
saccharification of cellulose to glucose and fermentation
of glucose to ethanol by a recombinant yeast strain as a
microbial biocatalyst. Recombinant yeast strain expressing
endoglucanase and β-glucosidase was able to produce
ethanol from β-glucan, CMC and acid swollen cellulose.
This indicates that the resultant yeast strain of this study
acts efficiently as a whole cell biocatalyst.
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