Reconstruction of a Rabbit Ulna Bone Defect Using Bone Marrow Stromal Cells and a PLA/β-TCP Composite by a Novel Sintering Method

Title
Reconstruction of a Rabbit Ulna Bone Defect Using Bone Marrow Stromal Cells and a PLA/β-TCP Composite by a Novel Sintering Method
Authors
정영미박민성김상수김병수김영하김상헌이진우김수현
Issue Date
2009-11
Publisher
Advanced engineering materials
Citation
VOL 11, NO 11, B169-B176
Abstract
In this study, we fabricated the PLA/b-TCP composites having a homogeneously interconnected porous structure without a skin layer by the novel sintering method, consisting of pressing and thermal treatment steps. BMSCs were seeded onto these scaffolds, and their ability was evaluated to promote osteogenic differentiation and bone tissue formation in vitro and in vivo. From the results, we confirmed that tissue ingrowth and bone-like tissue generation were more extensive in PLA/b-TCP cell-composite constructs produced by our novel sintering method than in constructs prepared from PLA alone or in constructs prepared by solvent casting. In addition, the sintered PLA/b-TCP cell-composite constructs exhibited higher ALP activity and calcium deposition than conventional solvent-cast cell-composite constructs. From the results, it is thought that in the PLA/b-TCP composites prepared with our novel sintering technique, the direct contact between b-TCP and the BMSCs may have more efficiently conducted osteogenicity signals to the BMSCs, as evidence by the increased ALP activity and enhanced calcium accumulation. Consequently, the results of this study demonstrate that biodegradable and osteogenic PLA/b-TCP composites fabricated by our novel sintering method may enhance bone regeneration and might be useful for bone tissue engineering.
URI
http://pubs.kist.re.kr/handle/201004/36596
ISSN
1438-1656
Appears in Collections:
KIST Publication > Article
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