Synthesis and enzymatic incorporation of allyl-based DNA sequencing-by-synthesis probes for 3'-O-mass tag analysis

Title
Synthesis and enzymatic incorporation of allyl-based DNA sequencing-by-synthesis probes for 3'-O-mass tag analysis
Authors
오광지남연희신동윤안대로
Keywords
Sequencing-by-synthesis; Mass tags; Polymerases; Primer extension; Reversible terminators
Issue Date
2009-11
Publisher
Bulletin of the Korean Chemical Society
Citation
VOL 30, NO 11, 2769-2772
Abstract
In summary, this paper presents the first result, to our knowledge, that describes sequencing-by-synthesis using reversible 3’-O-blocking groups as mass tags for DNA sequencing. Our mono-labeled nucleotide-based SBS method can potentially contribute to address the read length problems currently existing in the SBS technology. Detailed mass analysis results will be also reported in near future Spectral data of compound 5: 1H-NMR (300 MHz, D2O) δ 8.37 (s, 1H, 2-H), 8.09 (s, 1H, 8-H), 6.33 (brm, 1’-H), 4.93 (s, 1H, one of CH2(CH3)C=CH2), 4.86 (s, 1H, one of CH2(CH3) C=CH2), 4.42 (m, 1H, 3’-H), 4.30 (m, 1H, 4’-H), 4.02 (m, 2H, 5’-H), 3.95 (s, 2H, CH2(CH3)C=CH2), 2.60-2.72 (m, 2H, 2’-H), 1.63 (s, 3H, CH2(CH3)C=CH2); 31P NMR (242.6 MHz, D2O) δ ‒8.8 (d, J = 21.1 Hz, 1P, γ-P), ‒13.8 (d, J = 18.9 Hz, 1P, α-P), ‒24.9 (t, J = 20.6 Hz, 1P, β-P). MALDI-TOF MS (negative mode): calcd for C14H21N5O12P3 (M-H): 544, found: 544.
URI
http://pubs.kist.re.kr/handle/201004/36629
ISSN
0253-2964
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KIST Publication > Article
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