Structure and Characteristics of Novel Hydroxyapatite/Polylactide Bi-Layered Porous Scaffold for Bone Regeneration

Structure and Characteristics of Novel Hydroxyapatite/Polylactide Bi-Layered Porous Scaffold for Bone Regeneration
손준식Guda TejaMark ApplefordJoo L. Ong박귀덕한동근오선호
골 재생용; 하이드록시아파타이트; 폴리락티드; Hydroxyapatite; PLA; HEPM; bi-layered scaffold; bone regeneration
Issue Date
Tissue Engineering and Regenerative Medicine
VOL 6, NO 13, 1241-1251
The goal of this study was to develop novel hydroxyapatite (HAp)/ polylactide (PLA) bi-layered porous scaffold for tissue-engineered bone regeneration. To mimic normal bone structures, the outside cortical-like shells fabricated consisted of porous HAp and an inner trabecular-like core was made of porous PLA. Cortical-like porous HAp scaffolds with hollow in its inside was fabricated using a polymer sponge template. Trabecular-like porous PLA scaffold was directly formed by a particle leaching/gas foaming technique with sodium percarbonate as a novel porogen in inner diameter of HA scaffolds. The oxygen plasma treatment was performed to modify the PLA surface of bi-layered porous scaffold. The plasma-treated bi-layered scaffold was subsequently dipped in an alcoholic solution containing calcium and phosphate ions to deposit precursors on the surface. The surface modified bi-layered scaffold was immersed in simulated body fluid (SBF) at 5 times ionic concentration for 24 hr to obtain the final bonelike apatite coated HAp/PLA bi-layered porous scaffold. It was observed that the HAp and PLA parts of fabricated bi-layered scaffolds contain open and interconnected pore structures, with no structural delaminations on the external surfaces. Using helium pycnometry, the maximum porosity was observed to be greater than 96% for bi-layered scaffold. Additionally, the distribution of pore size for the PLA scaffolds was from 100 to 700 μm. Also, the dense and uniform bone-like apatite layer was successfully formed on surface of HAp/PLA bi-layered scaffold after immersion for 1 day in SBF solution. Total protein and alkaline phosphatase (ALP) content of bi-layered scaffold were higher than the control HAp scaffold during 4 weeks in vitro culture study using human embryonic palatal mesenchymal (HEPM) cells. It is indicated that bi-layered porous scaffold has stronger ability to induce HEPM cell attachment, prolife
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