Microbial Fed-batch Production of 1,3-Propanediol Using Raw Glycerol with Suspended and Immobilized Klebsiella pneumoniae

Title
Microbial Fed-batch Production of 1,3-Propanediol Using Raw Glycerol with Suspended and Immobilized Klebsiella pneumoniae
Authors
전선애문철우강철희공성욱상병인엄영순
Keywords
1,3-propanediol; glycerol; fermentation; Immobilization; Klebsiella pneumonia; Fed-batch; *2009년도 개인평가에 반영되었음
Issue Date
2010-05
Publisher
Applied biochemistry and biotechnology
Citation
VOL 161, NO 1-8, 491-501
Abstract
The production of 1,3-propanediol (1,3-PD) was investigated with Klebsiella pneumoniae DSM 4799 using raw glycerol without purification obtained from a biodiesel production process. Fed-batch cultures with suspended cells revealed that 1,3-PD production was more effective when utilizing raw glycerol than pure glycerol (productivity after 47 h of fermentation, 0.84 gL−1h−1 versus 1.51 gL−1h−1 with pure and raw glycerol, respectively). In addition, more than 80 g/L of 1,3-PD was produced using raw glycerol; this is the highest 1,3-PD concentration reported thus far for K. pneumoniae using raw glycerol. Repeated fed-batch fermentation with cell immobilization in a fixed-bed reactor was performed to enhance 1,3-PD production. Production of 1,3-PD increased with the cycle number (1.06 gL−1h−1 versus 1.61 gL−1h−1 at the first and fourth cycle, respectively) due to successful cell immobilization. During 46 cycles of fed-batch fermentation taking place over 1,460 h, a stable and reproducible 1,3-PD production performance was observed with both pure and raw glycerol. Based on our results, repeated fed batch with immobilized cells is an efficient fermentor configuration, and raw glycerol can be utilized to produce 1,3-PD without inhibitory effects caused by accumulated impurities.
URI
http://pubs.kist.re.kr/handle/201004/36735
ISSN
0273-2289
Appears in Collections:
KIST Publication > Article
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