The rapid identification of anthocyanins from blueberry, vaccinium corymbosum L. by LC-NMR and LC-MS
- The rapid identification of anthocyanins from blueberry, vaccinium corymbosum L. by LC-NMR and LC-MS
- 강석우; 김상민; 엄병헌
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- The anthocyanins and caffeoylquinic acid are major bioactive compounds in blue berry fruits, and the kinds and contents of anthocyanins are greatly changed in its cultivars and cultivation environment. In common, HPLC needs the standard compounds for the identification of these anthocyanins. In this study, the hyphenated LC-NMR and LC-MS techniques are applied for the direct identification of the major constituents present in buleberry. Reversed-phase chromatography was performed using an acetonitrile-water solvent system on a C18 column. The NMR spectrum yielded six main peaks, whose analysis revealed them to be delphinidin-3-galactoside (1), chlorogenic acid (2), delphinidin-3-arabinoside (3), petunidin-3-galactoside (4), malvidin-3-galactoside (5), and malvidin-3-arabinoside (6). The identities of these constituents were confirmed by performing LC-ESI/MS. This study shows that hyphenated LC-NMR and LC-MS can be used to rapidly (50 min) identify anthocyanins.
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