Phytoestrogenic effects of Disporum uniflorum in MCF-7 human breast cancer cells

Phytoestrogenic effects of Disporum uniflorum in MCF-7 human breast cancer cells
phytoestrogen; hormone replacement therapy; Disporum uniflorum; breast cancer; MCF-7; estrogen receptor
Issue Date
, 46-46
Phytoestrogens, natural compounds derived from plants, whose structures are similar with natural animal estrogen 17β-estradiol. So they are able to exert estrogenic effects both in human and animals. The main groups of phytoestrogen are isoflavones, lignans, coumestanes, stilbens and flavonoids. In this study, we investigated the estrogenic effect of various Korean wild vegetables. As a result of the screening, we found out that the ethanolic extract of Disporum uniflorum showed a strong estrogenic activity. We examined the estrogenic effects of the extract of D. uniflorum in MCF-7 human breast cancer cells as compared to genistein, a famous phytoestrogen. We discovered that the extract stimulates the proliferation of MCF-7 in hormone-starved condition by using MTT assay and clonogenic assay. The extract also induced the transcriptional activation of estrogen response element (ERE) in a dose-dependent manner. The extract at 20 μg/ml induced a 3.0-fold increase of ERE transactivation as compared to the vehicle control. We also measured the expression and phosphorylation of extracellular signal-regulated kinase (ERK) and Estrogen Receptor (ER) α, which are key factors regulated in estrogen signaling pathway by using western blot analysis. The extract increased the phosphorylation of ERK after 10 min, 24 h of treatment while the expression of total ERK was not changed. In addition, the extract induced the phosphorylation of ER α at Ser118 which is a important phosphorylation site of ER α triggered by the ligand binding. Especially, the treatment of the extract at the concentration of 40 μg/ml for 10 min strongly increased the phosphorylated of ER α at Ser118. In conclusion, the ethanolic extract of D. uniflorum showed a potent phytoestrogenic effect, therefore it might be developed as a functional food or natural medicine for the prevention and treatment of postmenoposal syndrome occurred in women.
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