Effect of pH and sulfate concentration on hydrogen production using anaerobic mixed microflora

Title
Effect of pH and sulfate concentration on hydrogen production using anaerobic mixed microflora
Authors
황재훈최정아R.A.I. Abou-ShanabAmit Bhatnagar민부기송호철Eva Kumar최재영이응석김용제엄석기이대성전병훈
Keywords
Hydrogen production; Anaerobic mixed microflora; pH; Sulfate; Fluorescence in situ hybridization
Issue Date
2009-12
Publisher
International journal of hydrogen energy
Citation
VOL 34, NO 24, 9702-9710
Abstract
The effects of varying sulfate concentrations with pH on continuous fermentative hydrogen production were studied using anaerobic mixed cultures growing on a glucose substrate in a chemostat reactor. The maximum hydrogen production rate was 2.8 L/day at pH 5.5 and sulfate concentration of 3000 mg/L. Hydrogen production and residual sulfate level decreased with increasing the pH from 5.5 to 6.2. The volatile fatty acids (VFAs) and ethanol fractions in the effluent were in the order of butyric acid (HBu) > acetic acid (HAc) > ethanol > propionic acid (HPr). Fluorescence In Situ Hybridization (FISH) analysis revealed the presence of hydrogen producing bacteria (HPB) under all pH ranges while sulfate reducing bacteria (SRB) were present at pH 5.8 and 6.2. The inhibition in hydrogen production by SRB at pH 6.2 diminished entirely by lowering to pH 5.5, at which activity of SRB is substantially suppressed.
URI
http://pubs.kist.re.kr/handle/201004/36884
ISSN
0360-3199
Appears in Collections:
KIST Publication > Article
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