A simple HPLC method for doxorubicin in plasma and tissues of nude mice
- A simple HPLC method for doxorubicin in plasma and tissues of nude mice
- Ahmed M. Al-Abd; 김남호; 송수창; 이승진; 구효정
- Doxorubicin; HPLC; Plasma; Tissue; Nude mice; Human tumor xenograft
- Issue Date
- Archives of pharmacal research
- VOL 32, NO 4, 605-611
Several HPLC methods have been reported for the quantification of doxorubicin in
biological samples. Tissue matrix effect and sample size requirements, however, have been
remaining issues for simple and easy-to-adapt analytical methods in small animal experiments.
The present study established a simple HPLC method for doxorubicin in plasma and
tissues (tumor, heart, spleen, liver, gastrointestinal tract, brain, lung, and kidney) of nude
mice. Our method required a small sample volume (100 μL plasma and 10 mg tissue), which
made it possible to use each blank tissue for calibration curves. The limit of quantification was
25 ng/mL in plasma and 0.1 to 0.4 μg/mg in other tissues with recovery rates ranging from
52.4 to 95.2%. The linearity, accuracy and precision in all tissues, except gastrointestinal tract
(GIT), were found to be acceptable in the range of 25-2000 ng/mL plasma and 0.1-4 ng/mg tissue.
This method was used successfully to determine the drug concentration in plasma and
tissues of human tumor xenograft-bearing nude mice given intratumoral doxorubicin in a
polymeric drug delivery system designed for sustained release. In conclusion, the present
method may be useful as a simple and easy-to-adapt, yet, sensitive analytical method of doxorubicin
for plasma and tissue pharmacokinetic studies in small animals such as nude mice.
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