Electrospray-Assisted Ultraviolet Aerodynamic Particle Sizer Spectrometer for Real-time Characterization of Bacterial Particles

Title
Electrospray-Assisted Ultraviolet Aerodynamic Particle Sizer Spectrometer for Real-time Characterization of Bacterial Particles
Authors
정재희이정은황기병이병욱이승복정종수배귀남
Keywords
electrospray; bioaerosols; nebulizer; UVAPS
Issue Date
2010-01
Publisher
Analytical chemistry
Citation
VOL 82, NO 2, 664-671
Abstract
The ultraviolet aerodynamic particle sizer (UVAPS) spectrometer is a novel, commercially available aerosol counter for real-time, continuous monitoring of viable bioaerosols based on the fluorescence induced from living microorganisms. For aerosolization of liquid-based microorganisms, general aerosolization methods such as atomization or nebulization may not be adequate for an accurate and quantitative characterization of the microorganisms because of the formation of agglomerated particles. In such cases, biological electrospray techniques have an advantage because they generate nonagglomerated particles, attributable to the repulsive electrical forces among particles with unipolar charges. Biological electrosprays are quickly gaining potential for the detection and control of living organisms in applications ranging from mass spectrometry to developmental microbiology. In this study, we investigated the size distribution, total concentration, and fluorescence percentage of bacterial particles in a real-time manner by electrospray-assisted UVAPS. A suspension containing Escherichia coli as a test microorganism was sprayed in a steady cone-jet mode using a specially designed electrospray system with a point-to-orifice-plate configuration based on charge-reduced electrospray size spectrometry. With the electrospray process, 98% of the total E. coli particle number concentration had a size of <1 μm and the geometric mean diameter was 0.779 μm, as compared with the respective values of 78% and 0.907 μm after nebulization. The fractions of fluorescence responsive particles and of particles that contained viable organisms in culture were 12% and 7%, respectively, from the electrospray process and 34% and 24% from nebulization.
URI
http://pubs.kist.re.kr/handle/201004/37080
ISSN
0003-2700
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE