Isolation and Characterization of Glycerol-Utilizing and Solventogenic Anaerobic Bacteria

Isolation and Characterization of Glycerol-Utilizing and Solventogenic Anaerobic Bacteria
Glycerol; Butanol; Fermentation
Issue Date
2010 International meeting of the microbiological society of Korea
Glycerol is a byproduct of bioethanol and biodiesel production processes and can be converted to more valuable products such as ethanol, butanol, 1,3-propanediol, and 2,3-butanediol by various anaerobic bacteria. In this study, we isolated glycerol-utilizing anaerobic bacteria to obtain ones having a superior ability to produce butanol. By adding acetic and butyric acids into the mineral medium containing glycerol, we could enrich butanol-producing bacteria from a soil sample. Isolates showed >99% 16S rRNA gene similarities with Clostridium diolis/beijerinckii (group 1), C. butyricum (group 2), C. arbusti (group 3), and Klebsiella oxytoca (group 4). The isolates belonging to group 3 produced up to 12.5 g/L of butanol and the isolates belonging to group 2 produced up to 17.1 g/L of 1,3-propanediol from 30 g/L of glycerol. When glucose was used instead of glycerol, the isolates belonging to group 1 produced 12.0 g/L of butanol and 6.9 g/L of ethanol and the isolate of group 4 produced 21.0 g/L of 2,3-butanediol and 8.0 g/L of ethanol from 60 g/L of glucose. This result shows the diversity of glycerol-utilizing anaerobic bacteria and their potential for industrial application.
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