Peptide-Grafted Lactide-Based Poly(ethylene glycol) Porous Scaffolds for Specific Cell Adhesion
- Peptide-Grafted Lactide-Based Poly(ethylene glycol) Porous Scaffolds for Specific Cell Adhesion
- 박철호; 홍유진; 박귀덕; 한동근
- tissue engineering; vascular stent; scaffold; peptide ligand; RGD; REDV
- Issue Date
- Macromolecular research
- VOL 18, NO 5, 526-532
- While biodegradable polyesters or their copolymers have been used widely as scaffolding materials in
tissue engineering, these polymers intrinsically lack the cell-binding motifs on the surface. In this study, a GL-PEG,
which is a poly(ethylene glycol) monoacrylate combined with glycerol (G)-lactide (L) triols, was synthesized and
fabricated into a porous three dimensional (3D) structure. In addition, peptide ligands, Arg-Gly-Asp (RGD) or Arg-
Glu-Asp-Val (REDV) were grafted directly onto the GL-PEG, producing GL-PEG-GRGD and GL-PEG-GREDV
scaffolds to improve the specific cell adhesion. Both GL-PEG and GL-PEG-GRDG scaffolds were also prepared as
a control. The ESCA spectra showed that peptide-grafted GL-PEG carried a nitrogen peak, which is indicative of
the amine group in the peptide sequence. The nitrogen content in the GL-PEG-peptides was significantly higher than
that on the the GL-PEG. When the peptide-grafted GL-PEG scaffolds were seeded with fibroblasts and endothelial
cells (ECs) and cultured for up to 4 days, specific cell interactions were identified by scanning electron microscopy
(SEM). More fibroblasts were found on the GL-PEG-GRGD scaffold but more ECs were found attached to GL-PEGGREDV.
The total protein assay also supported the same trend that the fibroblasts/GL-PEG-GRGD or EC/GL-PEGGREDV
constructs had the highest protein content with the specific cells, compared with the other groups. This data
suggests that cell adhesion could be specific and dependent on the type of peptide ligands. Such 3D porous and
crosslinked peptide-grafted GL-PEG scaffolds can be very useful for harboring specific cell populations for tissue
engineering and vascular stents.
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