Gymnasterkoreayne B protects against menadione-induced cytotoxicity by increasing quinone reductase through ARE-Nrf2 pathway in human hepatocarcinoma cells

Title
Gymnasterkoreayne B protects against menadione-induced cytotoxicity by increasing quinone reductase through ARE-Nrf2 pathway in human hepatocarcinoma cells
Authors
이샛별강경수사랑게렐조은혜윤지호유지혜이은하판철호이재권정상훈노주원
Issue Date
2010-05
Publisher
2010 한국생화학분자생물학회
Citation
, 258-258
Abstract
In the present study, we isolated gymnasterkoreayne B (GKB) from Gymnaster koraiensis and investigated the effect of GKB on the protection from oxidative stress-induced cytotoxicity through induction of the expression of cellular defense enzymes. GKB induced mRNA expression and enzyme activity of NAD(P)H:quinone oxidoreductase (NQO1) in vitro and in vivo, and increased the gene expression of many cellular defense genes including glutathione S-transferases, UDP glucuronosyltransferase, and glutathione reductase, from normal rat liver. The induction of NQO1, Heme oxygenase-1, and GSR by GKB was caused through the Nrf2-ARE (nuclear factor-E2-related factor 2- antioxidant response element) pathway in HepG2 human hepatocarcinoma cells. Pretreatment of the cells with GKB accelerated the production of glutathione (GSH) and mitigated menadione-induced cytotoxicity in HepG2 cells. Taken together, we found that GKB was a novel inducer of phase II detoxification enzymes and cellular defense enzymes, resulting in protection the cells from oxidative stress. Therefore, GKB could be developed as a potential cancer chemopreventive agent.
URI
http://pubs.kist.re.kr/handle/201004/37450
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KIST Publication > Conference Paper
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