Galactosylated Chitosan-g-PEI/DNA Complexes-loaded Poly(organophosphazene) Hydrogel as a Hepatocyte Targeting Gene Delivery System
- Galactosylated Chitosan-g-PEI/DNA Complexes-loaded Poly(organophosphazene) Hydrogel as a Hepatocyte Targeting Gene Delivery System
- Hu-Lin Jiang; 김유경; 이선미; 박미란; 김은미; 진용미; Rohidas Arote; 정환정; 송수창; 조명행; 조종수
- Gene therapy; Galactosylated chitosan-g-polyethylenimine; Poly(organophosphazene) hydrogel; Hepatocyte targeting; Cytotoxicity; Transfection efficiency
- Issue Date
- Archives of pharmacal research
- VOL 33, NO 4, 551-556
- Hydrogels are widely used in drug delivery systems because they can control the release and thereby enhance the efficiency of locally delivered bioactive molecules such as therapeutic drugs, proteins, or genes. For gene delivery, localized release of plasmid DNA or polymer/DNA complexes can transfect cells and produce sustained protein production. We tested the galactosylated chitosan-graft-polyethylenimine (GC-g-PEI)/DNA complexes-loaded poly(organophosphazene) thermosensitive biodegradable hydrogel as a hepatocyte targeting gene delivery system. The poly(organophosphazene) hydrogel loaded with GC-g-PEI/DNA complexes showed low cytotoxicity and higher transfection efficiency than PEI/DNA complexes, as well as good hepatocyte specificity in vitro and in vivo. Our results indicate that poly(organophosphazene) hydrogels loaded with GC-g-PEI/DNA complexes may be a safe and efficient hepatocyte targeting gene delivery system.
- Appears in Collections:
- KIST Publication > Article
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.