Galactosylated Chitosan-g-PEI/DNA Complexes-loaded Poly(organophosphazene) Hydrogel as a Hepatocyte Targeting Gene Delivery System

Title
Galactosylated Chitosan-g-PEI/DNA Complexes-loaded Poly(organophosphazene) Hydrogel as a Hepatocyte Targeting Gene Delivery System
Authors
Hu-Lin Jiang김유경이선미박미란김은미진용미Rohidas Arote정환정송수창조명행조종수
Keywords
Gene therapy; Galactosylated chitosan-g-polyethylenimine; Poly(organophosphazene) hydrogel; Hepatocyte targeting; Cytotoxicity; Transfection efficiency
Issue Date
2010-04
Publisher
Archives of pharmacal research
Citation
VOL 33, NO 4, 551-556
Abstract
Hydrogels are widely used in drug delivery systems because they can control the release and thereby enhance the efficiency of locally delivered bioactive molecules such as therapeutic drugs, proteins, or genes. For gene delivery, localized release of plasmid DNA or polymer/DNA complexes can transfect cells and produce sustained protein production. We tested the galactosylated chitosan-graft-polyethylenimine (GC-g-PEI)/DNA complexes-loaded poly(organophosphazene) thermosensitive biodegradable hydrogel as a hepatocyte targeting gene delivery system. The poly(organophosphazene) hydrogel loaded with GC-g-PEI/DNA complexes showed low cytotoxicity and higher transfection efficiency than PEI/DNA complexes, as well as good hepatocyte specificity in vitro and in vivo. Our results indicate that poly(organophosphazene) hydrogels loaded with GC-g-PEI/DNA complexes may be a safe and efficient hepatocyte targeting gene delivery system.
URI
http://pubs.kist.re.kr/handle/201004/37612
ISSN
0253-6269
Appears in Collections:
KIST Publication > Article
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