Hinokitiol activates the hypoxia-inducible factor (HIF) pathway through inhibition of HIF hydroxylases

Title
Hinokitiol activates the hypoxia-inducible factor (HIF) pathway through inhibition of HIF hydroxylases
Authors
이명진김정원양은경
Keywords
Hypoxia-inducible factor; Prolyl-4-hydroxylase; Factor-inhibiting HIF-1α; Vascular endothelial growth factor; Hinokitiol
Issue Date
2010-05
Publisher
Biochemical and biophysical research communications
Citation
VOL 396, NO 2, 370-375
Abstract
Hypoxia-inducible factor (HIF)-1 has been established as a master regulator of vascular responses to hypoxia and ischemia by driving transcriptional activation of angiogenic factors. Oxygen- and 2-oxoglutarate (2-OG)-dependent, iron(II) containing HIF-specific prolyl-4-hydroxylases (PHDs) and factor inhibiting HIF-1α (FIH-1) catalyze the hydroxylation of the specific proline and asparagine residues of HIF-1α, thereby controlling the level of HIF-1α and ultimately the HIF response. Here, we unveil a new action of hinokitiol, an iron chelator found in natural plants, on stabilization of HIF-1α in cell cultures in a dosedependent manner. In vitro PHD2 and FIH activity assays based on fluorescence polarization reveal that such HIF-1α stabilization is likely medicated by inhibitory effects of hinokitiol on prolyl and asparaginyl hydroxylation of HIF-1α. In addition, the inhibition of PHD2 by hinokitiol is reversed by the addition of 2- OG and iron(II), suggesting that the underlying inhibitory mechanism involves displacement of 2-OG and a chelate formation with iron(II) at the enzyme active site by hinokitiol. Furthermore, hinokitiol treated cells show increased transcription of vascular endothelial growth factor, providing the therapeutic potential in the treatment of ischemic diseases.
URI
http://pubs.kist.re.kr/handle/201004/37863
ISSN
1090-2104
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KIST Publication > Article
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