Production of minicellulosomes from Clostridium cellulovorans for the fermentation of cellulosic ethanol using engineered recombinant Saccharomyces cerevisiae

Title
Production of minicellulosomes from Clostridium cellulovorans for the fermentation of cellulosic ethanol using engineered recombinant Saccharomyces cerevisiae
Authors
현정은유경옥서동진서영웅이성은이진원한성옥
Keywords
minicellulosome; Clostridium cellulovorans; Saccharomyces cerevisiae; cellulose-binding domain; consolidated bioprocessing; ethanol fermentation
Issue Date
2010-09
Publisher
FEMS microbiology letters
Citation
VOL 310, NO 1, 39-47
Abstract
Saccharomyces cerevisiae was engineered for assembly of minicellulosomes by heterologous expression of a recombinant scaffolding protein from Clostridium cellulovorans and a chimeric endoglucanase E from Clostridium thermocellum. The chimeric endoglucanase E fused with the dockerin domain of endoglucanase B from C. cellulovorans was assembled with the recombinant scaffolding protein. The resulting strain was able to ferment amorphous cellulose [carboxymethyl-cellulose (CMC)] into ethanol with the aid of β-glucosidase 1 produced from Saccharomycopsis fibuligera. The minicellulosomes assembled in vivo retained the synergistic effect for cellulose hydrolysis. The minicellulosomes containing the cellulosebinding domain were purified by crystalline cellulose affinity in a single step. In the fermentation test at 10 g L-1 initial CMC, approximately 3.45 g L-1 ethanol was produced after 16 h. The yield (in grams of ethanol produced per substrate) was 0.34 g g-1 from CMC. This result indicates that a one-step processing of cellulosic biomass in a consolidated bioprocessing configuration is technically feasible by recombinant yeast cells expressing functional minicellulosomes.
URI
http://pubs.kist.re.kr/handle/201004/37892
ISSN
0378-1097
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KIST Publication > Article
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