Preparation of TGF-β1-conjugated biodegradable pluronic F127 hydrogel and its application with adipose-derived stem cells
- Preparation of TGF-β1-conjugated biodegradable pluronic F127 hydrogel and its application with adipose-derived stem cells
- 정홍희; 박귀덕; 한동근
- hydrogel; stem cells; Pluronic F127; Chondrogenesis; Human adipose-derived stem cell; Conjugation; TGF-β1 release
- Issue Date
- Journal of controlled release
- VOL 147, 84-91
- In this study, a composite hydrogel using Pluronic F127 derivatives and crosslinked hyaluronic acid (X-HA)
was investigated, exploring the benefits in the induction of chondrogenic differentiation of human adiposederived
stem cells (ASCs). F127 was chemically modified through a series of reactions that produced
multiple F127 derivatives. A chondrogenic growth factor, transforming growth factor-beta 1 (TGF-β1) was
then coupled to the heparin-conjugated F127. X-HA was used as a physical stabilizer of the composite
hydrogel (X-HA/F127). The chemical structures of F127 derivatives were analyzed using 1H-NMR and ATRFTIR.
Sol–gel transition of the composite hydrogel was identified at body temperature. The conjugated TGF-
β1 was moderately released in vitro from the composite hydrogel. Cell viability of human ASCs in the
hydrogels was about 50% after in vitro culture for 3 days. As the ASCs/hydrogel were injected into nude mice
subcutaneously, DAPI staining of the retrieved constructs showed that ASCs were dispersed through the
hydrogel matrix. From the immunofluorescent staining of type II collagen, the TGF-conjugated group
exhibited more active green signals than the others. In addition, when those constructs were loaded into the
full-thickness defect of rabbit knee articular cartilage, Alcian blue staining identified the formation of
cartilaginous matrix from the TGF-conjugated hydrogel. The present work indicated that X-HA/F127
composite hydrogel was thermoreversible and biodegradable, and that the TGF-conjugated hydrogel could
be effective in inducing chondrogenesis of human ASCs.
- Appears in Collections:
- KIST Publication > Article
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.