B. licheniformis and C. tyrobutyricum co-culture for butyric acid production : characterization of B. licheniformis SGP1 strain and studying the growth pattern of the two strains in the co-culture

Title
B. licheniformis and C. tyrobutyricum co-culture for butyric acid production : characterization of B. licheniformis SGP1 strain and studying the growth pattern of the two strains in the co-culture
Authors
드위달김세일전병승엄영순상병인
Keywords
Clostridium tyrobutyricum; butyric acid; sucrose; co-culture; Bacillus licheniformis
Issue Date
2010-10
Publisher
2010년도 한국생물공학회 추계학술발표대회
Abstract
In a previous study, we co-cultured the novel strain Bacillus licheniformis SGP1 with Clostridium tyrobutyricum ATCC 25755 for butyric acid production using sucrose as a carbon source in RCM medium. In this co-culture, B. licheniformis produces levansucrase enzyme in the medium which degrades sucrose into monosaccharides allowing C. tyrobutyricum to utilize them along with the lactic acid produced by B. licheniformis for butyric acid production. In this study, we wanted to determine the effect of different factors on the growth of this novel Bacillus strain and its levansucrase production and activity. Optimum pH was around 7.0. Optimum temperature for growth is 37℃. Enzyme production is decreased dramatically as the temperature rises from 30 ℃ to 43 ℃ and the reverse happens for enzyme activity. This enzyme has a molecular weight of 55 KDa. In fed-batch fermentation at pH 5.50 and temperature of 37 ℃, the co-culture could give a final butyrate concentration of 36 g/L with productivity equals 0.24 g/L/hr, while at 40 ℃, we could get 28.6 g/L and 0.34 g/L/hr respectively. Real time PCR experiment was done finally to determine the growth pattern of the two strains in the co-culture.
URI
http://pubs.kist.re.kr/handle/201004/38028
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KIST Publication > Conference Paper
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