Co-culturing Bacillus lichinoformis with Clostridium tyrobutyricum for production of butyric acid from sucrose

Title
Co-culturing Bacillus lichinoformis with Clostridium tyrobutyricum for production of butyric acid from sucrose
Authors
드위달김세일박재연전병승엄영순상병인
Keywords
clostridium tyrobutyricum; butyric acid; sucrose; co-culture; Bacillus lichinoformis
Issue Date
2010-05
Publisher
2010 international meeting of the microbiological society of Korea
Abstract
Butyric acid has several potential applications in chemical industries and food technology. Currently, the most promising microorganism used for the bio-production of butyric acid is Clostridium tyrobutyricum. In this study, a strain of Bacillus lichinoformis isolated from waste water sludge was used in a co-culture with C.tyrobutyricum ATCC 25755 in serum bottles containing RCM supplemented with sucrose as the sole carbon source for the production of butyric acid. Although C.tyrobutyricum alone is unable to use sucrose as a carbon source, this co-culture was able to use sucrose and to produce butyric acid in large concentration (up to 10 g/l) which is even higher than that produced from C.tyrobutyricum alone in serum bottles when glucose is used as a carbon source under the same conditions. B.lichinoformis alone did not show any butyric acid production. Implying that B.lichinoformis degrades sucrose into monosaccharides by specific enzymes and then C.tyrobutyricum uses them for butyric acid production, butyric acid was the main product in this co-culture. Also, the final acetic acid concentration in the medium was significantly lower than that produced from C.tyrobutyricum alone when glucose is used as a carbon source. In addition, this co-culture could grow in the RCM broth in serum bottles without initial purging with inert gas for providing anaerobic conditions. We conclude here that the mentioned co-culture has a possible potential to be applied for butyric acid production from sucrose on industrial scale to decrease the overall cost.
URI
http://pubs.kist.re.kr/handle/201004/38029
Appears in Collections:
KIST Publication > Conference Paper
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE