Co-culturing Bacillus lichinoformis with Clostridium tyrobutyricum for production of butyric acid from sucrose
- Co-culturing Bacillus lichinoformis with Clostridium tyrobutyricum for production of butyric acid from sucrose
- 드위달; 김세일; 박재연; 전병승; 엄영순; 상병인
- clostridium tyrobutyricum; butyric acid; sucrose; co-culture; Bacillus lichinoformis
- Issue Date
- 2010 international meeting of the microbiological society of Korea
- Butyric acid has several potential applications in chemical industries and food technology. Currently, the most promising microorganism used for the bio-production of butyric acid is Clostridium tyrobutyricum. In this study, a strain of Bacillus lichinoformis isolated from waste water sludge was used in a co-culture with C.tyrobutyricum ATCC 25755 in serum bottles containing RCM supplemented with sucrose as the sole carbon source for the production of butyric acid. Although C.tyrobutyricum alone is unable to use sucrose as a carbon source, this co-culture was able to use sucrose and to produce butyric acid in large concentration (up to 10 g/l) which is even higher than that produced from C.tyrobutyricum alone in serum bottles when glucose is used as a carbon source under the same conditions. B.lichinoformis alone did not show any butyric acid production. Implying that B.lichinoformis degrades sucrose into monosaccharides by specific enzymes and then C.tyrobutyricum uses them for butyric acid production, butyric acid was the main product in this co-culture. Also, the final acetic acid concentration in the medium was significantly lower than that produced from C.tyrobutyricum alone when glucose is used as a carbon source. In addition, this co-culture could grow in the RCM broth in serum bottles without initial purging with inert gas for providing anaerobic conditions. We conclude here that the mentioned co-culture has a possible potential to be applied for butyric acid production from sucrose on industrial scale to decrease the overall cost.
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