In situ thermal gelling polypeptide for chondrocytes 3D culture
- In situ thermal gelling polypeptide for chondrocytes 3D culture
- 최보규; 박민희; 조소혜; 주민경; 오혜진; 김은혜; 박귀덕; 한동근; 정병문
- polypeptide; 3D culture; thermal gelling; Secondary structure; Peptide; Thermally responsive material; Hydrogel; Chondrocyte; Cell culture
- Issue Date
- VOL 31, NO 35, 9266-9272
- In the search for a cell-instructive or cell-interactive artificial extracellular matrix, synthetic hydrogels
have been extensively investigated to apply three-dimensional (3D) cell culture and tissue engineering.
Here, we are reporting a reverse thermal gelling L/DL-polyalanine block copolymer aqueous solution for
chondrocyte 3D culture. The polymer aqueous solution undergoes sol-to-gel transition as the temperature
increases, thus forming a 3D cell encapsulating scaffold in situ at 37 ℃. In particular, the fraction of
the β-sheet structure of the polyalanine dictated the population and thickness of fibrous nanostructure of
the hydrogel, which in turn affected the proliferation and protein expression of the encapsulated
chondrocytes. As an injectable tissue engineering system of chondrocytes, very promising results were
confirmed for nude mice, using the current polypeptide aqueous solution. This paper not only provides
important clues in designing an artificial extracellular matrix but also proves the significance of thermal
gelling polypeptide as a minimally-invasive tissue engineering scaffold.
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