Hepatoprotective effects of Actinidia arguta against oxidative stress induced tert-butyl hydroperoxide
- Hepatoprotective effects of Actinidia arguta against oxidative stress induced tert-butyl hydroperoxide
- 조은혜; 강경수; 노주원
- Actinidia arguta; Hepatoprotection; Acetaminophene; oxidative stress
- Issue Date
- 2010 한국응용생명화학회
- , 390-390
- The fruits and leaves of Actinidia arguta are a well-known Korea folk medicine for treatment of jaundice, fever, acute hepatitis and bleeding by an external wound. The purpose of this study was to investigate the potential hepatoprotective effects of Actinidia arguta on tert-Butyl hydroperoxide (t-BHP) induced oxidative stress in HepG2 human liver cancer cells. Cell viability, generation of reactive oxygen species, total polyphenolic compounds were evaluated in this study. Pretreatment of HepG2 cells with various concentrations of Actinidia arguta extract (EXT) strongly prevented cytotoxicity from the cells. We also measured Hexane (H), Ethyl acetate (EA), Butanol (B) fraction. Reactive oxygen species generation induced by t-BHP was significantly decreased when the cells were pretreated for 24 h with either 10-40 μg/ml EXT or H, EA, B fraction. In addition, we measured protective effects of EXT and 3 fractions against cytotoxicity induced Acetaminophene. The contents of total polyphenolic compounds were determined using a modified Folin-Ciocalteu colorimetric method. Especially, B fraction (161.52 mg/100g) showed the highest contents of total polyphenolic compounds which could affect the potent hepatoprotective activity. Therefore, these results suggest that the Actinidia arguta could be a potential candidate of hepatoprotective agents against oxidative stress.
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