Tumor targeting chitosan nanoparticles for dual-modality optical/MR cancer imaging
- Tumor targeting chitosan nanoparticles for dual-modality optical/MR cancer imaging
- 남태환; 박상진; 이승영; 박경순; 최귀원; 송인찬; 한문희; James J.Leary; Simseok Andrew Yuk; 권익찬; 김광명; 정서영
- Issue Date
- Bioconjugate chemistry
- VOL 21, NO 4, 578-582
- We report tumor targeting nanoparticles for optical/MR dual imaging based on self-assembled glycol chitosan to
be a potential multimodal imaging probe. To develop an optical/MR dual imaging probe, biocompatible and
water-soluble glycol chitosan (Mw = 50 kDa) were chemically modified with 5β-cholanic acid (CA), resulting in
amphiphilic glycol chitosan-5β-cholanic acid conjugates (GC-CA). For optical imaging near-infrared fluorescence
(NIRF) dye, Cy5.5, was conjugated to GC-CA resulting in Cy5-labeled GC-CA conjugates (Cy5.5-GC-CA).
Moreover, in order to chelate gadolinium (Gd(III)) in the Cy5.5-GC-CA conjugates, 1,4,7,10-tetraazacyclododecane-
1,4,7,10-tetraacetic acid (DOTA) was directly conjugated in Cy5.5-GC-CA. Finally, the excess GdCl3 was added
to DOTA modified Cy5.5-GC-CA conjugates in distilled water (pH 5.5). The freshly prepared Gd(III) encapsulated
Cy5.5-GC-CA conjugates were spontaneously self-assembled into stable Cy5.5 labeled and Gd(III) encapsulated
chitosan nanoparticles (Cy5.5-CNP-Gd(III)). The Cy5.5-CNP-Gd(III) was spherical in shape and approximately
350 nm in size. From the cellular experiment, it was demonstrated that Cy5.5-CNP-Gd(III) were efficiently taken
up and distributed in cytoplasm (NIRF filter; red). When the Cy5.5-GC-Gd(III) were systemically administrated
into the tail vein of tumor-bearing mice, large amounts of nanoparticles were successfully localized within the
tumor, which was confirmed by noninvasive near-infrared fluorescence and MR imaging system simultaneously.
These results revealed that the dual-modal imaging probe of Cy5.5-CNP-Gd(III) has the potential to be used as
an optical/MR dual imaging agent for cancer treatment.
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