Cellular uptake pathway and drug release characteristics of drug-encapsulated glycol chitosan nanoparticles in live cells

Title
Cellular uptake pathway and drug release characteristics of drug-encapsulated glycol chitosan nanoparticles in live cells
Authors
박상진이소진정현진허송욱최용석김광명최귀원권익찬
Keywords
hydrophobically modified glycol chitosan; nanoparticle; cellular uptake; endocytosis; intracellular trafficking; cellular imaging
Issue Date
2010-09
Publisher
Microscopy research and technique
Citation
VOL 73, NO 9, 857-865
Abstract
Herein, we evaluated the cellular uptake pathways of hydrophobically modified glycol chitosan (HGC) nanoparticles as nano-sized drug carriers using cellular imaging technology. The endocytic pathway of nanocarriers for intracellular drug delivery is of great interest for the design of high efficacy delivery carriers for therapeutic agents. To evaluate the cellular uptake pathways of HGC nanoparticles, HGC was chemically labeled with near infrared (NIR) fluorescence dye, Cy5.5, to visualize the nanoparticle under confocal laser scanning microscopy. The internalization pathways of HGC nanoparticles were evaluated after treatment of specific endocytosis inhibitors. Importantly, HCG nanoparticles showed different cellular uptake efficiency and intracellular fate in cytoplasm according to the internalization pathways. Furthermore, drug distribution also evaluated according to the endocytic pathways after treatment of drug encapsulated HGC nanoparticles. As a model drug, fluorescent photosensitizer, Ce6, was encapsulated into HGC (Ce6- HGC) nanoparticles and the distribution of Ce6 in cytoplasm was evaluated using confocal laser scanning microscopy. The intracellular drug distribution showed different manner through specific endocytic pathways. The cellular imaging technology is highly useful for evaluation of endocytosis pathways and intracellular fate of drug delivery carrier which are closely related to drug distribution and therapeutic efficacy.
URI
http://pubs.kist.re.kr/handle/201004/38160
ISSN
1059-910X
Appears in Collections:
KIST Publication > Article
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