Cellular uptake pathway and drug release characteristics of drug-encapsulated glycol chitosan nanoparticles in live cells
- Cellular uptake pathway and drug release characteristics of drug-encapsulated glycol chitosan nanoparticles in live cells
- 박상진; 이소진; 정현진; 허송욱; 최용석; 김광명; 최귀원; 권익찬
- hydrophobically modified glycol chitosan; nanoparticle; cellular uptake; endocytosis; intracellular trafficking; cellular imaging
- Issue Date
- Microscopy research and technique
- VOL 73, NO 9, 857-865
- Herein, we evaluated the cellular uptake pathways of hydrophobically modified
glycol chitosan (HGC) nanoparticles as nano-sized drug carriers using cellular imaging technology.
The endocytic pathway of nanocarriers for intracellular drug delivery is of great interest for the
design of high efficacy delivery carriers for therapeutic agents. To evaluate the cellular uptake
pathways of HGC nanoparticles, HGC was chemically labeled with near infrared (NIR) fluorescence
dye, Cy5.5, to visualize the nanoparticle under confocal laser scanning microscopy. The internalization
pathways of HGC nanoparticles were evaluated after treatment of specific endocytosis
inhibitors. Importantly, HCG nanoparticles showed different cellular uptake efficiency and intracellular
fate in cytoplasm according to the internalization pathways. Furthermore, drug distribution
also evaluated according to the endocytic pathways after treatment of drug encapsulated HGC
nanoparticles. As a model drug, fluorescent photosensitizer, Ce6, was encapsulated into HGC (Ce6-
HGC) nanoparticles and the distribution of Ce6 in cytoplasm was evaluated using confocal laser
scanning microscopy. The intracellular drug distribution showed different manner through specific
endocytic pathways. The cellular imaging technology is highly useful for evaluation of endocytosis
pathways and intracellular fate of drug delivery carrier which are closely related to drug distribution
and therapeutic efficacy.
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