Pegylated poly-L-arginine derivatives of chitosan for effective delivery of siRNA

Title
Pegylated poly-L-arginine derivatives of chitosan for effective delivery of siRNA
Authors
노상명박명옥심가영한수은이한영허준혁김명숙최진주김광명권익찬김진석백광현오유경
Keywords
Chitosan; Polyarginine; Polyethylene glycol; siRNA; Serum stability
Issue Date
2010-07
Publisher
Journal of controlled release
Citation
VOL 145, NO 2, 159-164
Abstract
For delivery of siRNA, chitosan (CS) was derivatized with poly-L-arginine (PLR) and polyethylene glycol (PEG). The formation of polyplexes with siRNA was confirmed by gel retardation. The PLR-grafted CS formed nanosized particles with siRNA. PLR-grafted CS showed higher cellular delivery efficiency of siRNA than did CS, pegylated CS, PLR, or pegylated PLR. The extent of reduction in the expression of fluorescent proteins was highest following treatment of the cells using PLR derivatives of CS in complexes with specific siRNAs. Cell viability was greater in populations treated with pegylated CS–PLR than in those treated with PLR. Hemolysis of erythrocytes was reduced upon conjugation of PLR with CS. The delivery of siRNAs via pegylated CS–PLR revealed little dependence on serum. Molecular imaging techniques revealed that the intratumoral administration of red fluorescent protein-specific siRNA in complexes with pegylated CS–PLR significantly silenced the expression of red fluorescent proteins in tumor tissues in vivo. These results indicate that pegylated CS–PLR might be useful for in vivo delivery of therapeutic siRNAs.
URI
http://pubs.kist.re.kr/handle/201004/38208
ISSN
0168-3659
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KIST Publication > Article
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