Effects of Folic Acid and Polyethylene Glycol Conjugated Quantum Dot on Toxicity and Tissue Uptake to Precision-cut Spleen Slices of Rats
- Effects of Folic Acid and Polyethylene Glycol Conjugated Quantum Dot on Toxicity and Tissue Uptake to Precision-cut Spleen Slices of Rats
- 마무눌 하크; 락쉬미 데비 칸다가다라; 임혜연; 우경자; 권오승
- quantum dot nanoparticles; CdSe/CdS; carboxylic acid; polyethylene
- Issue Date
- 2010 Proceedings of the Fall International Convention of The Pharmaceutical Society of Korea
- , 137-137
- Nano-sized materials are increasingly used in cosmetics, diagnosis, imaging, and drug delivery. It also involved in specific functionality with lymphoid systems. However, the toxicity and mechanisms of semiconductor nanoparticles (quantum dot, QD) uptake into cells are poorly understood. Our study was to investigate toxicity and tissue uptake of folic acid-polyethylene glycol-conjugated (FA-PEG), and only polyethylene glycol-conjugated (PEG) cadmium selenide/cadmium sulfide (CdSe/CdS) QDs using precision-cut spleen slices of rats. QDs were treated with different doses (0-300 nM) to the spleen of Sprague-Dawley rats, and their effects on toxicity and tissue uptake were examined by LDH, NADPH oxidase activity, and histological analysis. No dose-dependent changes in LDH were observed. But high uptake of QD-PEG-FA into spleen slices was observed by fluorescence microscopic examination in dose-dependent manner, while most of QD-PEG was found on the edge of the slices. NADPH oxidase activity was increased at high dose (300 nM) in QD-PEG-FA- or QD-PEG-treated spleen slices indicating oxidative stresses. No damages were noticed in histological study confirming no toxicity in both types of QDs. These findings provide insight into the specific mechanism of QD uptake in cells. Based on the above observations, we conclude that surface coating property, and/or charge of QD nanoparticles are important factors in determining nanoparticle uptake in mammalian cells.
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