Suppression of Glutamate-Induced Excitotoxicity by 2-Cyclopropylimino-3-methyl-1,3-thiazoline Hydrochloride in Rat Glial Cultures

Title
Suppression of Glutamate-Induced Excitotoxicity by 2-Cyclopropylimino-3-methyl-1,3-thiazoline Hydrochloride in Rat Glial Cultures
Authors
Eun-A kim한호규김기선Tae Ue KimSoo Young ChoiSung-Woo Cho
Keywords
Glutamate; Cytokines; Calcium; Nitric oxide; β-Catenin; GSK-3 beta; 2-Cyclopropylimino-3-methyl-1; 3-thiazoline hydrochloride; GSK-3β
Issue Date
2010-07
Publisher
Cellular and molecular neurobiology
Citation
VOL 30, NO 5, 807-815
Abstract
We have screened new drugs with a view to developing effective drugs against glutamate-induced excitotoxicity. In the present work, we show effects of a new drug, 2-cyclopropylimino-3-methyl-1,3-thiazoline hydrochloride against glutamate-induced excitotoxicity in primary rat glial cultures. Pretreatment of glial cells with 2-cyclopropylimino-3-methyl-1,3-thiazoline hydrochloride for 2 h significantly protected glial cells against glutamateinduced excitotoxicity in a time- and dose-dependent manner with an optimum concentration of 100 lM. The drug significantly reduced production of proinflammatory cytokines, tumor necrosis factor-α, and interlukin-1β in glutamate-induced excitotoxicity. The drug also prevented glutamate-induced intracellular Ca2+ influx and reduced the subsequent overproduction of nitric oxide and reactive oxygen species. Furthermore, the drug preserved the mitochondrial potential and inhibited the overproduction of cytochrome c. In addition, the drug effectively attenuated the protein level changes of β-catenin and glycogen synthase kinase-3β. These results suggest that 2-cyclopropylimino- 3-methyl-1,3-thiazoline hydrochloride effectively protected primary cultures of rat glial cells against glutamate- induced excitotoxicity.
URI
http://pubs.kist.re.kr/handle/201004/38273
ISSN
0272-4340
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE