Articular cartilage tissue engineering based on a mechano-active scaffold made of poly(L-lactide-co-ε-caprolactone): In vivo performance in adult rabbits

Title
Articular cartilage tissue engineering based on a mechano-active scaffold made of poly(L-lactide-co-ε-caprolactone): In vivo performance in adult rabbits
Authors
Jun XieZhiyi HanMasatoshi NaitoAkira Maeyama김수현김영하Takehisa Matsuda
Keywords
cartilage; tissue engineering; mechano-active scaffold
Issue Date
2010-07
Publisher
Journal of biomedical materials research. Part B, Applied biomaterials
Citation
VOL 94B, NO 1, 80-88
Abstract
Our previous studies showed that a mechano-active scaffold made of poly(L-lactide-co-ε-caprolactone) (PLCL) exhibited a high potential to realize the formation of a functional, engineered cartilage in vitro. This animal study therefore was designed to investigate the feasibility of repairing on osteochondral defect with the use of bone marrow-derived mesenchymal stem cells (BMSCs) incorporated with a PLCL scaffold. Rabbit BMSCs, isolated and subsequently cultured in monolayer, were seeded into a porous PLCL scaffold sponge following an implantation onto a full-thickness osteochondral defect (diameter of 4.5 mm, depth of 5 mm) that was artificially created on the medial femoral condyles at a high loadbearing site on a rabbit’s knee joint. Time-dependent healing of the defect was evaluated by macroscopic, histological examinations at both 3- and 6-month-implantations, respectively. A PLCL sponge incorporated with BMSCs exhibited sufficient structural support, resulting in new osteochondral tissue regeneration: a physiologically well-integrated subchondral bone formation, a hyaline cartilage-like morphology containing chondrocytes surrounded by abundant cartilaginous matrices. In addition, quantitative biochemical assays also demonstrated high potential for the synthesis of sulfated glycosaminoglycan and collagen, both of which are biomolecules essential to extracelluar matrix in normal cartilage tissue. In contrast, defects filled with cell-free PLCL scaffold or left empty showed a very limited potential for regeneration. Our findings suggest that a composite of PLCL-based sponge scaffold and BMSCs promote the repair of osteochondral defects at high load-bearing sites in adult rabbits.
URI
http://pubs.kist.re.kr/handle/201004/38354
ISSN
1552-4973
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE