Sequence discrimination of the Zα domain of human ADAR1 during B-Z transition of DNA duplexes

Title
Sequence discrimination of the Zα domain of human ADAR1 during B-Z transition of DNA duplexes
Authors
서여진안희철이은해방종철강영민김희은이연미김경민최병석이준화
Keywords
NMR; Z-DNA; hydrogen exchange; Z-DNA binding protein; B-Z transition; Non-CG repeat DNA
Issue Date
2010-10
Publisher
FEBS letters
Citation
VOL 584, NO 20, 4344-4350
Abstract
The Zα domain of human ADAR1 (ZαADAR1) preferentially binds Z-DNA rather than B-DNA with high binding affinity. ZαADAR1 binds to the Z-conformation of both non-CG-repeat DNA duplexes and a d(CGCGCG)₂duplex similarly. We performed NMR experiments on complexes between the ZαADAR1 and non-CG-repeat DNA duplexes, d(CACGTG)₂or d(CGTACG)₂, with a variety of protein-DNA molar ratios. Comparison of these results with those from the analysis of d(CGCGCG)₂in the previous study suggests that ZαADAR1 exhibits the sequence preference of d(CGCGCG)₂》d(CACGTG)₂> d(CGTACG)₂ through multiple sequence discrimination steps during the B–Z transition.
URI
http://pubs.kist.re.kr/handle/201004/38360
ISSN
0014-5793
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KIST Publication > Article
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