Sequence discrimination of the Zα domain of human ADAR1 during B-Z transition of DNA duplexes
- Sequence discrimination of the Zα domain of human ADAR1 during B-Z transition of DNA duplexes
- 서여진; 안희철; 이은해; 방종철; 강영민; 김희은; 이연미; 김경민; 최병석; 이준화
- NMR; Z-DNA; hydrogen exchange; Z-DNA binding protein; B-Z transition; Non-CG repeat DNA
- Issue Date
- FEBS letters
- VOL 584, NO 20, 4344-4350
- The Zα domain of human ADAR1 (ZαADAR1) preferentially binds Z-DNA rather than B-DNA with high
binding affinity. ZαADAR1 binds to the Z-conformation of both non-CG-repeat DNA duplexes and a
d(CGCGCG)₂duplex similarly. We performed NMR experiments on complexes between the ZαADAR1
and non-CG-repeat DNA duplexes, d(CACGTG)₂or d(CGTACG)₂, with a variety of protein-DNA molar
ratios. Comparison of these results with those from the analysis of d(CGCGCG)₂in the previous study
suggests that ZαADAR1 exhibits the sequence preference of d(CGCGCG)₂》d(CACGTG)₂> d(CGTACG)₂
through multiple sequence discrimination steps during the B–Z transition.
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