NMR study of hydrogen exchange during the B-Z transition of a DNA duplex induced by the Zα domains of yatapoxvirus E3L

Title
NMR study of hydrogen exchange during the B-Z transition of a DNA duplex induced by the Zα domains of yatapoxvirus E3L
Authors
이은해서여진안희철강영민김희은이연미최병석이준화
Keywords
NMR; Z-DNA; hydrogen exchange; Z-DNA binding protein; E3L; Poxvirus
Issue Date
2010-10
Publisher
FEBS letters
Citation
VOL 584, NO 21, 4453-4457
Abstract
The Yaba-like disease viruses (YLDV) are members of the Yatapoxvirus family and have doublestranded DNA genomes. The E3L protein, which is essential for pathogenesis in the vaccinia virus, consists of two domains: an N-terminal Z-DNA binding domain and a C-terminal RNA binding domain. The crystal structure of the E3L orthologue of YLDV (yabZαE3L) bound to Z-DNA revealed that the overall structure of yabZαE3L and its interaction with Z-DNA are very similar to those of hZaADAR1. Here we have performed NMR hydrogen exchange experiments on the complexes between yabZαE3L and d(CGCGCG)₂with a variety of protein-to-DNA molar ratios. This study revealed that yabZαE3L could efficiently change the B-form helix of the d(CGCGCG)₂to left-handed Z-DNA via the active-mono B–Z transition pathway like hZαADAR1.
URI
http://pubs.kist.re.kr/handle/201004/38362
ISSN
0014-5793
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KIST Publication > Article
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