NMR study of hydrogen exchange during the B-Z transition of a DNA duplex induced by the Zα domains of yatapoxvirus E3L
- NMR study of hydrogen exchange during the B-Z transition of a DNA duplex induced by the Zα domains of yatapoxvirus E3L
- 이은해; 서여진; 안희철; 강영민; 김희은; 이연미; 최병석; 이준화
- NMR; Z-DNA; hydrogen exchange; Z-DNA binding protein; E3L; Poxvirus
- Issue Date
- FEBS letters
- VOL 584, NO 21, 4453-4457
- The Yaba-like disease viruses (YLDV) are members of the Yatapoxvirus family and have doublestranded
DNA genomes. The E3L protein, which is essential for pathogenesis in the vaccinia virus,
consists of two domains: an N-terminal Z-DNA binding domain and a C-terminal RNA binding
domain. The crystal structure of the E3L orthologue of YLDV (yabZαE3L) bound to Z-DNA revealed
that the overall structure of yabZαE3L and its interaction with Z-DNA are very similar to those of
hZaADAR1. Here we have performed NMR hydrogen exchange experiments on the complexes between
yabZαE3L and d(CGCGCG)₂with a variety of protein-to-DNA molar ratios. This study revealed that
yabZαE3L could efficiently change the B-form helix of the d(CGCGCG)₂to left-handed Z-DNA via the
active-mono B–Z transition pathway like hZαADAR1.
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