Expression and activation of matrix metalloproteinase-9 and NADPH oxidase in tissue and plasma of experimental autoimmune encephalomyelitis in mice

Title
Expression and activation of matrix metalloproteinase-9 and NADPH oxidase in tissue and plasma of experimental autoimmune encephalomyelitis in mice
Authors
락쉬미 데비 칸다가다라강민정정봉철Tucker A Patterson권오승
Keywords
MMP-9; NADPH oxidase; EAE; CNS tissue; Spleen; Mice
Issue Date
2012-01
Publisher
Experimental and toxicologic pathology
Citation
VOL 64, NO 1-2, 109-114
Abstract
Experimental autoimmune encephalomyelitis (EAE) is a widely used animal model for multiple sclerosis (MS) that can be induced by immunization with myelin antigens such as myelin oligodendrocyte glycoprotein (MOG). The objective of this study was (i) to investigate how matrix metalloproteinase-9 (MMP-9) and NADPH oxidase enzymes are affected in the EAE mouse model and (ii) to know whether peripheral organs also express these enzymes in the EAE model. MOG33–55 was administered subcutaneously on two sites over the back. Pertussis toxin was administered intraperitoneally immediately after MOG and again two days later. A significant difference was observed in body weights and clinical signs of EAE-induced mice. MMP-9 and NADPH oxidase enzymes were measured in central nervous system (CNS) tissues, peripheral tissues and plasma of EAE-induced mice. The primary findings include the distribution pattern of MMP-9 in CNS and peripheral tissues, and alterations in the enzymatic expression of MMP-9 and NADPH oxidase in the CNS tissues, spleen and plasma of EAE-induced mice. From these results, it can be considered that the spleen as well as the CNS can act as target organs in EAE disease, and plasma MMP-9 and NADPH oxidase may contribute to the pathogenesis of the disease.
URI
http://pubs.kist.re.kr/handle/201004/38374
ISSN
0940-2993
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE