Aeromicrobium halocynthiae sp. nov., a taurocholic acid-producing bacterium isolated from the marine ascidian Halocynthia roretzi

Title
Aeromicrobium halocynthiae sp. nov., a taurocholic acid-producing bacterium isolated from the marine ascidian Halocynthia roretzi
Authors
김성훈양현옥손영창권학철
Keywords
Aeromicrobium halocyntiae; marine bacterium; marine ascidian; Halocynthia roretzi
Issue Date
2010-12
Publisher
International journal of systematic and evolutionary microbiology
Citation
VOL 60, NO 12, 2793-2798
Abstract
A marine bacterium, strain KME 001T, was isolated from the siphon tissue of a marine ascidian, Halocynthia roretzi, collected off the coast of Gangneung, Korea. Strain KME 001T was a Grampositive, aerobic, non-spore-forming, rod-shaped and non-motile bacterium. Phylogenetic analysis using 16S rRNA gene sequences revealed that strain KME 001T clustered with the genus Aeromicrobium and was closely related to Aeromicrobium ginsengisoli, Aeromicrobium erythreum and Aeromicrobium ponti with 97.7, 97.6 and 97.5% sequence similarities, respectively. The strain was capable of growth at a variety of temperatures (10–42 6℃) and over a broad pH range (5.0–10.0). NaCl was required for robust growth of the strain. The diagnostic diamino acid of the cell-wall peptidoglycan was LL-diaminopimelic acid. The predominant menaquinone was MK-9(H4). The predominant fatty acids were C18 : 1ω9c, C16 : 0 and 10-methyl C18 : 0. The DNA–DNA hybridization analyses showed that DNA–DNA relatedness values between strain KME 001T and its nearest neighbours, A. ginsengisoli KCTC 19207T, A. erythreum KCCM 41104T and A. ponti KACC 20565T, were 49.6, 57.1 and 63.5 %, respectively. The DNA G+C content of strain KME 001T was 75.9 mol%. Chemical investigation of the liquid culture medium of strain KME 001T led to the isolation of taurocholic acid as a major secondary metabolite. On the basis of phylogenetic and phenotypic data, strain KME 001T is classified as representing a novel species of the genus Aeromicrobium, for which the name Aeromicrobium halocynthiae sp. nov. is proposed. The type strain is KME 001T (=JCM 15749T=KCCM 90079T).
URI
http://pubs.kist.re.kr/handle/201004/38428
ISSN
1466-5026
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KIST Publication > Article
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