Aeromicrobium halocynthiae sp. nov., a taurocholic acid-producing bacterium isolated from the marine ascidian Halocynthia roretzi
- Aeromicrobium halocynthiae sp. nov., a taurocholic acid-producing bacterium isolated from the marine ascidian Halocynthia roretzi
- 김성훈; 양현옥; 손영창; 권학철
- Aeromicrobium halocyntiae; marine bacterium; marine ascidian; Halocynthia roretzi
- Issue Date
- International journal of systematic and evolutionary microbiology
- VOL 60, NO 12, 2793-2798
- A marine bacterium, strain KME 001T, was isolated from the siphon tissue of a marine ascidian,
Halocynthia roretzi, collected off the coast of Gangneung, Korea. Strain KME 001T was a Grampositive,
aerobic, non-spore-forming, rod-shaped and non-motile bacterium. Phylogenetic
analysis using 16S rRNA gene sequences revealed that strain KME 001T clustered with the
genus Aeromicrobium and was closely related to Aeromicrobium ginsengisoli, Aeromicrobium
erythreum and Aeromicrobium ponti with 97.7, 97.6 and 97.5% sequence similarities,
respectively. The strain was capable of growth at a variety of temperatures (10–42 6℃) and over a
broad pH range (5.0–10.0). NaCl was required for robust growth of the strain. The diagnostic
diamino acid of the cell-wall peptidoglycan was LL-diaminopimelic acid. The predominant
menaquinone was MK-9(H4). The predominant fatty acids were C18 : 1ω9c, C16 : 0 and 10-methyl
C18 : 0. The DNA–DNA hybridization analyses showed that DNA–DNA relatedness values
between strain KME 001T and its nearest neighbours, A. ginsengisoli KCTC 19207T, A.
erythreum KCCM 41104T and A. ponti KACC 20565T, were 49.6, 57.1 and 63.5 %, respectively.
The DNA G+C content of strain KME 001T was 75.9 mol%. Chemical investigation of the liquid
culture medium of strain KME 001T led to the isolation of taurocholic acid as a major secondary
metabolite. On the basis of phylogenetic and phenotypic data, strain KME 001T is classified as
representing a novel species of the genus Aeromicrobium, for which the name Aeromicrobium
halocynthiae sp. nov. is proposed. The type strain is KME 001T (=JCM 15749T=KCCM 90079T).
- Appears in Collections:
- KIST Publication > Article
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.