Neuroprotective effect of modified Wu-Zi-Yan-Zong ganule, a traditional Chinese herbal medicine, on CoCl2-induced PC12 cells
- Neuroprotective effect of modified Wu-Zi-Yan-Zong ganule, a traditional Chinese herbal medicine, on CoCl2-induced PC12 cells
- 쩡꿔위; Wang, Xue-Mei; 고현석; 양현옥
- Neuroprotection; Modified Wu-Zi-Yan-Zong granule; Traditional Chinese medicine; PC12 cells; Cobalt chloride
- Issue Date
- Journal of ethnopharmacology
- VOL 130, NO 1, 13-18
- Aim of the study: To investigate the neuroprotective effect of aqueous extract of modified Wu-Zi-Yan-Zong
granule (MWG), a traditional Chinese herbal medicine, against CoCl2-induced neurotoxicity in PC12 cells.
Materials and methods: Cell viability assay, apoptosis rate assay, ROS detection and mitochondrial membrane
potential (MMP) assay were performed. In addition, cytochrome c, caspase-3, PARP and MAPKs
were also detected by Western blotting.
Results: MWG extract increased viability and suppresses early and middle/late stage apoptosis in a
dose-dependent manner in CoCl2-induced PC12 cells. Moreover, MWG extract decreased the level of
intracellular reactive oxygen species (ROS), increased MMP, regulated Bcl-2 family protein expression
(Bcl-2 and Bcl-XL) and inhibited the release of cytochrome c from the mitochondria. In addition, MWG
extract attenuated activation of caspase-3 and poly ADP-ribose polymerase (PARP) and inhibited the
phosphorylation of ERK, c-Jun NH2-terminal kinase (JNK) and p38 MAPKs.
Conclusions: MWG extract exhibited significant neuroprotective effect on PC12 cells, and this effect may
be associated with the suppression of ROS generation and inhibition of mitochondria-mediated caspase
and MAPK signaling pathways.
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- KIST Publication > Article
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