Neuroprotective effect of modified Wu-Zi-Yan-Zong ganule, a traditional Chinese herbal medicine, on CoCl2-induced PC12 cells

Title
Neuroprotective effect of modified Wu-Zi-Yan-Zong ganule, a traditional Chinese herbal medicine, on CoCl2-induced PC12 cells
Authors
쩡꿔위Wang, Xue-Mei고현석양현옥
Keywords
Neuroprotection; Modified Wu-Zi-Yan-Zong granule; Traditional Chinese medicine; PC12 cells; Cobalt chloride
Issue Date
2010-07
Publisher
Journal of ethnopharmacology
Citation
VOL 130, NO 1, 13-18
Abstract
Aim of the study: To investigate the neuroprotective effect of aqueous extract of modified Wu-Zi-Yan-Zong granule (MWG), a traditional Chinese herbal medicine, against CoCl2-induced neurotoxicity in PC12 cells. Materials and methods: Cell viability assay, apoptosis rate assay, ROS detection and mitochondrial membrane potential (MMP) assay were performed. In addition, cytochrome c, caspase-3, PARP and MAPKs were also detected by Western blotting. Results: MWG extract increased viability and suppresses early and middle/late stage apoptosis in a dose-dependent manner in CoCl2-induced PC12 cells. Moreover, MWG extract decreased the level of intracellular reactive oxygen species (ROS), increased MMP, regulated Bcl-2 family protein expression (Bcl-2 and Bcl-XL) and inhibited the release of cytochrome c from the mitochondria. In addition, MWG extract attenuated activation of caspase-3 and poly ADP-ribose polymerase (PARP) and inhibited the phosphorylation of ERK, c-Jun NH2-terminal kinase (JNK) and p38 MAPKs. Conclusions: MWG extract exhibited significant neuroprotective effect on PC12 cells, and this effect may be associated with the suppression of ROS generation and inhibition of mitochondria-mediated caspase and MAPK signaling pathways.
URI
http://pubs.kist.re.kr/handle/201004/38430
ISSN
0378-8741
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KIST Publication > Article
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