Phylogenetic Characterization of the Microbial Community in Pilot-scale Membrane Bioreactor Plants by 454-Pyrosequencing

Phylogenetic Characterization of the Microbial Community in Pilot-scale Membrane Bioreactor Plants by 454-Pyrosequencing
Membrane bioreactor; Biofouling; Pyrosequencing; Microbial community
Issue Date
Membranes in Drinking and Industrial Water Treatment (MDIW) 2010
Membrane bioreactor (MBR) process is a pressure-driven separation process which has been extensively used in areas of wastewater treatment and reuse. However, the sharp reduction of the flux far below the theoretical capacity mainly due to membrane biofouling which was typically caused by undesirable sorption of colloids, extracellular polymeric substances (EPS), and bacteria on the surface and internal pore of the membrane has become the detrimental problem in the efficiency and economics of the membrane separation process. Based on the literature review, little has been known about the microbial community in the biofoulants on the fouled membrane as well as the sludge in the MBR plants. Therefore, this study is focusing on the phylogenetic characterization of the microbial communities and the phylogenetic relationship between the biofoulants and the sludge liquors in two pilot-scale MBR plants in South Korea. The pylogenetic structure of the microbial community residing in samples obtained from pilot-scale membrane bioreactor (MBR) plants was analyzed by an integrated approach using clone library sequences and metagenome sequence data obtained by 454-pyrosequencing method. In order to identify metagenome reads carrying 16-rRNA sequence information, a homology search to the RDP database by means of BLASTn was conducted. Aligned read sequences were extracted from the dataset and further analysed by means of BLASTn with the low complexity filter turned off. Significant hits to the set of the 109 16S-rDNA amplicon sequences obtained during this study with an alignment length of at least 60 bases were classified according to the observed identity values (95~100%). All reads that could not be assigned to any of the amplicon sequences were grouped into OTUs according to their nearest neighbor (best matching BLAST-hit) in the RDP database. Only BLAST hits with an e-value below 1e−5, hit-length above 50 bp and pai
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