The emerging process of Top Down mass spectrometry for protein analysis: biomarkers, protein-therapeutics, and achieving high throughput
- The emerging process of Top Down mass spectrometry for protein analysis: biomarkers, protein-therapeutics, and achieving high throughput
- John F. Kellie; John C. Tran; 이지은; Dorothy R. Ahlf; Haylee M. Thomas; Ioanna Ntai; Adam D. Catherman; Kenneth R. Durbin; Leonid Zamdborg; Adaikkalam Vellaicha; Paul M. Thomas; Neil L. Kelleher
- Top Down mass spectrometry; proteomics
- Issue Date
- Molecular bioSystems.
- VOL 6, NO 9, 1532-1539
- Top Down mass spectrometry (MS) has emerged as an alternative to common Bottom Up
strategies for protein analysis. In the Top Down approach, intact proteins are fragmented directly
in the mass spectrometer to achieve both protein identification and characterization, even
capturing information on combinatorial post-translational modifications. Just in the past two
years, Top Down MS has seen incremental advances in instrumentation and dedicated software,
and has also experienced a major boost from refined separations of whole proteins in complex
mixtures that have both high recovery and reproducibility. Combined with steadily advancing
commercial MS instrumentation and data processing, a high-throughput workflow covering intact
proteins and polypeptides up to 70 kDa is directly visible in the near future.
- Appears in Collections:
- KIST Publication > ETC
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.