The emerging process of Top Down mass spectrometry for protein analysis: biomarkers, protein-therapeutics, and achieving high throughput

Title
The emerging process of Top Down mass spectrometry for protein analysis: biomarkers, protein-therapeutics, and achieving high throughput
Authors
John F. KellieJohn C. Tran이지은Dorothy R. AhlfHaylee M. ThomasIoanna NtaiAdam D. CathermanKenneth R. DurbinLeonid ZamdborgAdaikkalam VellaichaPaul M. ThomasNeil L. Kelleher
Keywords
Top Down mass spectrometry; proteomics
Issue Date
2010-09
Publisher
Molecular bioSystems.
Citation
VOL 6, NO 9, 1532-1539
Abstract
Top Down mass spectrometry (MS) has emerged as an alternative to common Bottom Up strategies for protein analysis. In the Top Down approach, intact proteins are fragmented directly in the mass spectrometer to achieve both protein identification and characterization, even capturing information on combinatorial post-translational modifications. Just in the past two years, Top Down MS has seen incremental advances in instrumentation and dedicated software, and has also experienced a major boost from refined separations of whole proteins in complex mixtures that have both high recovery and reproducibility. Combined with steadily advancing commercial MS instrumentation and data processing, a high-throughput workflow covering intact proteins and polypeptides up to 70 kDa is directly visible in the near future.
URI
http://pubs.kist.re.kr/handle/201004/38603
ISSN
1742-206X
Appears in Collections:
KIST Publication > ETC
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE