Production of hexanoic acid from D-galactitol by a newly isolated Clostridium sp. BS-1

Production of hexanoic acid from D-galactitol by a newly isolated Clostridium sp. BS-1
Clostridium sp. BS-1; Sludge; Hexanoic acid; D-galactitol; Coculture; *2010년 기관고유사업평가에 반영됨
Issue Date
Applied microbiology and biotechnology
VOL 88, NO 5, 1161-1167
In a study screening anaerobic microbes utilizing D-galactitol as a fermentable carbon source, four bacterial strains were isolated from an enrichment culture producing H2, ethanol, butanol, acetic acid, butyric acid, and hexanoic acid. Among these isolates, strain BS-1 produced hexanoic acid as a major metabolic product of anaerobic fermentation with D-galactitol. Strain BS-1 belonged to the genus Clostridium based on phylogenetic analysis using 16S rRNA gene sequences, and the most closely related strain was Clostridium sporosphaeroides DSM 1294T, with 94.4% 16S rRNA gene similarity. In batch cultures, Clostridium sp. BS-1 produced 550±31 mLL−1 of H2, 0.36±0.01 gL−1 of acetic acid, 0.44±0.01 gL−1 of butyric acid, and 0.98±0.03 gL−1 of hexanoic acid in a 4-day cultivation. The production of hexanoic acid increased to 1.22 and 1.73 gL−1 with the addition of 1.5 gL−1 of sodium acetate and 100 mM 2-(N-morpholino)ethanesulfonic acid (MES), respectively. Especially when 1.5 gL−1 of sodium acetate and 100 mM MES were added simultaneously, the production of hexanoic acid increased up to 2.99 gL−1. Without adding sodium acetate, 2.75 gL−1 of hexanoic acid production from D-galactitol was achieved using a coculture of Clostridium sp. BS-1 and one of the isolates, Clostridium sp. BS-7, in the presence of 100 mM MES. In addition, volatile fatty acid (VFA) production by Clostridium sp. BS- 1 from D-galactitol and D-glucose was enhanced when a more reduced culture redox potential (CRP) was applied via addition of Na2S·9H2O.
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