Hypnotic effects and binding studies for GABAA and 5-HT2C receptors of traditional medicinal plants used in Asia for insomnia

Title
Hypnotic effects and binding studies for GABAA and 5-HT2C receptors of traditional medicinal plants used in Asia for insomnia
Authors
조승목Makoto Shimizu이창준한대석정철균조진호김영명
Keywords
Oriental medicinal plant; Hypnotic activity; GABAA receptor; 5-HT2C receptor; Licorice (Glycyrrhiza uralensis Fischer); Silk tree (Albizzia julibrissin Durazzini)
Issue Date
2010-10
Publisher
Journal of ethnopharmacology
Citation
VOL 132, NO 1, 225-232
Abstract
Aim of the study: Many medicinal plants have been used for treatment of insomnia in Asia. However, scientific evidence and precise mechanism for their sedative-hypnotic activity have not been fully investigated. Thus, we investigated the binding activity of the oriental plant extracts (mainly from Korea and Japan) to the well-known molecular targets for sleep regulation, GABAA and 5-HT2C receptors. Following the binding assay, sedative-hypnotic effects of the extracts with high affinity were examined in an animal model of sleep. Materials and methods: Aqueous and ethanol extracts of 15 medicinal plants were tested for binding at the benzodiazepine site of GABAA receptor and 5-HT site of 5-HT2C receptor. The sedative-hypnotic effects of selected extracts were evaluated by measuring the sleep latency and sleep duration during pentobarbital-induced sleep in mice after oral administration of extracts. Results: In the GABAA assay, the ethanol extracts of licorice and danshen displayed concentrationdependent, high affinity binding, whereas in the 5-HT2C assay, the ethanol extracts of ginseng and silk tree showed high affinity. Among these extracts we tested previously uncharacterized licorice and silk tree for hypnotic effects. We found the ethanol extracts of licorice and silk tree significantly decreased sleep latency and increased sleep duration in pentobarbital-induced sleep. Conclusions: We demonstrate for the first time that licorice and silk tree have the sedative-hypnotic activity possibly by modulating GABAA and 5-HT2C receptors. We propose that licorice and silk tree might be effective candidates for treatment of insomnia.
URI
http://pubs.kist.re.kr/handle/201004/38616
ISSN
0378-8741
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KIST Publication > Article
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