Generation of core-shell microcapsules with three-dimensional focusing device for efficient formation of cell spheroid
- Generation of core-shell microcapsules with three-dimensional focusing device for efficient formation of cell spheroid
- 김충; 정석; 김영은; 이강선; 이수현; 오광욱; 강지윤
- microcapsule; focusing; cell spheroid; stem cell
- Issue Date
- Lab on a chip
- VOL 11, NO 2, 246-252
- We present a microfluidic device generating three-dimensional (3D) coaxial flow by the addition of
a simple hillock to produce an alginate core-shell microcapsule for the efficient formation of a cell
spheroid. A hillock tapered at downstream of the two-dimensional focusing channel enables outside
flow to enclose the core flow. The aqueous solution in the core flow was focused and surrounded by
1.8% alginate solution to be solidified as a shell. The double-layered coaxial flow (aqueous phase) was
broken up into a droplet by the shear flow of oleic acid (oil phase) containing calcium chloride for the
polymerization of the alginate shell. The droplet generated from the laminar coaxial flow maintained
a double-layer structure and gelation of the alginate solution made a core-shell microcapsule. The shellthickness
of the microcapsule was adjusted from 8–21 mm by the variation of two aqueous flow rates.
The inner shape of the shell was almost spherical when the ratio of the water–glycol mixture in the core
flow exceeded 20%. The microcapsule was used to form a spheroid of embryonic carcinoma cells
(embryoid body; EB) by injecting a cell suspension into the core flow. The cells inside the microcapsule
aggregated into an EB within 2 days and the EB formation rate was more than 80% with strong
compaction. The microcapsule formed single spherical EBs without small satellite clusters or a bumpy
shape as observed in solid microbeads. The microfluidic chip for encapsulation of cells could generate
a number of EBs with high rate of EB formation when compared with the conventional hanging drop
method. The core-shell microcapsule generated by 3D focusing in the microchannel was effective in
forming large number of spherical cell clusters and the encapsulation of cells in the microcapsule is
expected to be useful in the transplantation of islet cells or cancer stem cell enrichment.
- Appears in Collections:
- KIST Publication > Article
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.