A proteomic approach based on multiple parallel separation for the unambiguous identification of an antibody cognate antigen

Title
A proteomic approach based on multiple parallel separation for the unambiguous identification of an antibody cognate antigen
Authors
문주희김용학유종한배진희노동영유명희이철주
Keywords
Autoantibody; Multiple parallel separation; Tumor-associated antigens
Issue Date
2010-10
Publisher
Electrophoresis
Citation
VOL 31, NO 20, 3428-3436
Abstract
Autoantibodies obtained from cancer patients have been identified as useful tools for cancer diagnostics, prognostics, and as potential targets for immunotherapy. Serological proteome analysis in combination with 2-DE is a classic strategy for identification of tumor-associated antigens in the serum of cancer patients. However, serological proteome analysis cannot always indicate the true antigen out of a complex proteome identified from a single protein spot because the most abundant protein is not always the most antigenic. To address this problem, we utilized multiple parallel separation (MPS) for proteome separation. The common identities present in the fractions obtained using different separation methods were regarded as the true antigens. The merit of our MPS technique was validated using anti-ARPC2 and anti-PTEN antibodies. Next, we applied the MPS technique for the identification of glycyl-tRNA synthetase as the cognate antigen for an autoantibody that was overexpressed in the plasma of breast cancer patients. These results reveal that MPS can unambiguously identify an antibody cognate antigen by reducing false-positives. Therefore, MPS could be used for the characterization of diagnostic antibodies raised in laboratory animals as well as autoantibodies isolated from diseased patients.
URI
http://pubs.kist.re.kr/handle/201004/38645
ISSN
0173-0835
Appears in Collections:
KIST Publication > Article
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