EFFECT of SMOOTH MUSCLE CELL-DERIVED ECM on ENDOTHELIAL CELL BEHAVIOR
- EFFECT of SMOOTH MUSCLE CELL-DERIVED ECM on ENDOTHELIAL CELL BEHAVIOR
- 이양기; 박귀덕; 한동근
- Endothelial cell; Extracellular matrix; decellularization; microenviroment
- Issue Date
- 2010 International Symposium of Materials on Regenerative Medicine
- extracellular matrix (ECM) is a physical entity that is a complex and highly ordered array of cell-derived proteins and other biomolecules. The hierarchical structure of these components provides a natural environment in which cells can survive and function properly. Therefore. many studies utilize ECM as a valuable resource for tissue engineering and regenerative medicine. The primary interest of this work is to prepare naturally derived ECM from smooth muscle cells (SMCs) and to evaluate their effects on attachment and proliferation of endothelial cells (ECs) as well as SMCs. Our hypothesis is that SMC-derived matrix (SDM) may function as a good substratum of ECs. because m the body both ECs and SMCs would interact closely each other. Once SMCs were cultured for a while, the cellular components were removed using a decellularization solution. The degree of decellularization was examined using DAPI staining. Surface morphology of SDM was observed using SEM. To identify the constituents within the SDM. immunofluorescent staining (IFS) was also carried out using primary Abs and Alexa Fluor 488 or 594 conjugated-secondary Abs for fibronectin (FN) and type I collagen (Coi I), respectively. After ECs and SMCs were individually seeded onto the SDM, cell proliferation was tested using a cell counting kit-8. Once decellularize SDM exhibited many fibrils in varying sizes. DAPI staining showed that the cellular conponents were completely removed. IFS uncovered that SDM retained a rather randomly distributed structure of FN. ECs proliferation was favorabIe with the SDM. Further study is expected to reveal the benefits of using SDM as a substrate of ECs for vascular grafts and stents.
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