Effective chondrogenic differentiation of stem cell in RGD-grafted Pluronic F127 hydrogel
- Effective chondrogenic differentiation of stem cell in RGD-grafted Pluronic F127 hydrogel
- 이혜선; 이봉수; 최보규; 박귀덕; 정병문; 한동근
- Issue Date
- 2010 한국고분자학회 추계학술대회
- Thermogelling Pluronic F127 hydrogel approved by the FDA has been studied extensively for drug delivery system and tissue engineering as a biomaterial for human applications. For improving cell adhension and biodegradability FGM-RGD was synthesized by covalent bonding of RGD peptides to F127-glycolide-4-methacryl-oxyethyl trimellitic anhydride (FGM), where Pluronic F127 derivatives have novel temperature-sensitive properties. These polymers were characterized by 1H NMR, FTIR, GPC, and elemental analyzer. In the cell viability study, the optical density (OD) of cell in FGM gel was resulted in very low value. It was revealed from cell counter kit-8(CCK-8) that the toxicity increased as the content of APS/TEMED increased, and resulted in inhibiting the cell viability of chondrocytes. In order to enhance cell viability in hydrogel, COOH group-blocking FGM and RGD-grafted FGM will be synthesized and characterized.
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