Effect of medium composition on butanol and 1,3-propanediol production by Clostridium pasteurianum DSM525

Title
Effect of medium composition on butanol and 1,3-propanediol production by Clostridium pasteurianum DSM525
Authors
문철우상병인엄영순
Keywords
butanol; 1,3-propanediol; glycerol
Issue Date
2010-11
Publisher
AIChE (American Institute of Chemical Engineering, 미국화학공학회)
Abstract
In this study, the effect of glycerol concentration, butanol concentration, co-substrates, and pH on product formation by Clostridium pasteurianum DSM525 was investigated. Glycerol concentration higher than 60 g/L inhibited cell growth and butanol production. When more than 8 g/L of butanol concentraion was added initially, C. pateurianum DSM525 did not grow at all. The addition of butyrate was found to enhance butanol production by more than 50%, while there was no significant effect of acetate on butanol production. When pH-buffering agent was added to prevent over-acidification during the batch cultivation, butanol production was higher than 1,3-PD production. Without MES addition, however, butanol concentration was lower than 1,3-PD. The medium composition for butanol productivity by C. pasteurianum DSM525 was optimized using response surface methodology (RSM). Three factors (FeSO4·7H2O, yeast extract, (NH4)2SO4) were identified to affect butanol production significantly on the basis of a fractional factorial design (FFD). Using Box-Behnken Model (BBM), the optimal composition for three factors was determined to be 0.06 g/L of FeSO4·7H2O, 7.4 g/L of (NH4)2SO4 and 5.0 g/L of yeast extract. The predicted butanol productivity value by RSM analysis (0.98 g L-1h-1) was verified with the experiment result (1.01 g L-1h-1).
URI
http://pubs.kist.re.kr/handle/201004/38681
Appears in Collections:
KIST Publication > Conference Paper
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