Effect of medium composition on butanol and 1,3-propanediol production by Clostridium pasteurianum DSM525
- Effect of medium composition on butanol and 1,3-propanediol production by Clostridium pasteurianum DSM525
- 문철우; 상병인; 엄영순
- butanol; 1,3-propanediol; glycerol
- Issue Date
- AIChE (American Institute of Chemical Engineering, 미국화학공학회)
- In this study, the effect of glycerol concentration, butanol concentration, co-substrates, and pH on
product formation by Clostridium pasteurianum DSM525 was investigated. Glycerol concentration
higher than 60 g/L inhibited cell growth and butanol production. When more than 8 g/L of butanol
concentraion was added initially, C. pateurianum DSM525 did not grow at all. The addition of butyrate
was found to enhance butanol production by more than 50%, while there was no significant effect of
acetate on butanol production. When pH-buffering agent was added to prevent over-acidification during
the batch cultivation, butanol production was higher than 1,3-PD production. Without MES addition,
however, butanol concentration was lower than 1,3-PD. The medium composition for butanol
productivity by C. pasteurianum DSM525 was optimized using response surface methodology (RSM).
Three factors (FeSO4·7H2O, yeast extract, (NH4)2SO4) were identified to affect butanol production
significantly on the basis of a fractional factorial design (FFD). Using Box-Behnken Model (BBM), the
optimal composition for three factors was determined to be 0.06 g/L of FeSO4·7H2O, 7.4 g/L of
(NH4)2SO4 and 5.0 g/L of yeast extract. The predicted butanol productivity value by RSM analysis (0.98
g L-1h-1) was verified with the experiment result (1.01 g L-1h-1).
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