Adhesion behavior of non-specific cells on wrinkled, dual-scaled diamond-like carbon (DLC) superhydrophobic surface

Adhesion behavior of non-specific cells on wrinkled, dual-scaled diamond-like carbon (DLC) superhydrophobic surface
Yudi Rahmawan강도현이광렬문명운서갑양
Wrinkle; cell growth; superhydrophobic; cells adhesion; diamond-like carbon; anti-fouling
Issue Date
International Symposium on Nature Inspired Technology(ISNIT2010)
The textured surface with superhydrophobic nature was explored for calf pulmonary artery endothelial (CPAE), human breast normal (MCF-10A), and cancer (MCF-7) cells adhesion template. Hierarchical structures composed of the nano-scale wrinkle covering on micro-scale polymer pillar patterns were fabricated by combining the deposition of a thin coating layer of biocompatible diamond-like carbon (DLC) and the replica molding of poly-(dimethylsiloxane) (PDMS) micro-pillars. The resulting surface assembly consists of micro-scale PDMS pillars covered by nano-scale wrinkles that are induced by a residual compressive stress and a difference in elastic moduli between DLC and PDMS without any external stretching or thermal contraction on the PDMS substrate. In addition to providing the mechanical conditions on wrinkle formation, one-step DLC coating also brings a chemical functionality of low surface energy or higher water wetting angle on the surface with hierarchical structure, which enhances superhydrophobicity. A mathematical model of wetting states on dual-scale structures is derived by decoupling of nano- and microscale roughness contribution. The as-prepared surfaces were shown to have extreme hydrophobicity (static contact angle > 160 o) owing to low surface energy (24.2 mN/m) and dual-roughness structures of the DLC coating. It was explored that the hierarchical surfaces showed poor adhesion of the CPAE and MCF-10A cells for cultures of 7 days suggesting that the wrinkled, dual-scaled DLC superhydrophobic surface exhibits excellent anti-biofouling properties against non-specific normal cell adhesion. In particular, the reduced filopodia extension during cell growth was caused by disconnected focal adhesions on the pillar pattern. This limited cell adhesion could prevent undesired growth and proliferation of biological species on the surface of biomedical devices such as stents, implants or even injection syringes. T
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