Protein cages provide a platform of cell-permeable and biocompatible imaging probe in living cells
- Protein cages provide a platform of cell-permeable and biocompatible imaging probe in living cells
- 최승혜; 최귀원; 권익찬; 황광연; 안형준
- 분자영상; 케이지 단백질; 세포사멸; 플래폼 기술; 프로브
- Issue Date
- The 10th Conference of the Asia Crystallographic Association
- , 401-401
- Caged proteins are macromolecules exhibiting a self-assembled nanoplatform that can be used for detection of biomolecule. We present here the strategy to image apoptotic cells using a nanoplatform of caged protein. For that purpose, cage protein was genetically modified to have two abilities, real-time imaging of apoptosis and cellualr uptake. A high ratio of target peptides to cage formed from self-assembling of cage subints resulted in increase of fluorescence signals per a probe particle, and our cage probe was well cell-permeable and exceedingly biocompatible. We demonstrated that cege probe was specifically cleaved by effector caspase in cell-free conditions, and also finally apoptotic events were imaged real-timely in living cells. Our strategy provides an application to imaging and sensing of other specific protease activities.
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