Baicalin protects retinal ischemia and oxidative insults to retinal cells

Title
Baicalin protects retinal ischemia and oxidative insults to retinal cells
Authors
정상훈
Keywords
Baicalin; retinal ischemia; retinal ganglion cells; glaucoma
Issue Date
2010-02
Publisher
Symposium on the Application of Natural Products for Age-related Diseases
Abstract
This study was to determine whether baicalin isolated from Scutellaria baicalensis couldattenuate oxidative stress induced transformed retinal ganglion cells (RGC-5 cells) death.RGC-5 cells in culture were given one ofthl-ee different insults, light (1000 lux for 48hours), hydrogen peroxide (200 u.M HzOz for 24 hours), or serum deprivation (48 hours),after which cell survival and reactive oxygen species (ROS) formation were assayedMoreover, a lipid peroxidation assay was used to compare the antioxidant capacity of thecompounds with epigallocatechin gallate (EGCG). In this study, baicalin attenuated thenegative insult of light, hydrogen peroxide, or serum withdrawal to RGC-5 cells.Treatment of the RGC-5 cells with baicalin reduced the reactive oxygen species (ROS)by using the fluorescence probe, 2',7'-dich1orodihydrof1uorescein diacetate (DCFH -DA), and decreased glutathione level by using ELISA kit. Moreover, baicalin attenuatedapoptosis caused by three different models of oxidative stress measured by propidiumiodide (PI)/Hoechst 33342 double staining and APOVercentageU kit assay.In the animal model, ischemia/reperfusion to the retina afFected the localisation of Thy-1 and choline acetyltransferase (ChAT) and the content of various proteins (optic nerveand retina) and mRNAs (retina). Importantly, baicalin statistically blunted most of theeffects induced by ischemia/reperfusion.In conclusion, these results indicate that baicalin could be offered as leading compoundsfor retinal diseases via antioxidative and antiapoptotic effects.
URI
http://pubs.kist.re.kr/handle/201004/38826
Appears in Collections:
KIST Publication > Conference Paper
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