ELECTRICALLY TETHERED DNA STRETCHING IN NANOCHANNELS

Title
ELECTRICALLY TETHERED DNA STRETCHING IN NANOCHANNELS
Authors
이병철강찬민김진식강지윤신현준이상엽
Issue Date
2009-11
Publisher
ASME International Mechanical Engineering Congress and Exposition
Abstract
In present study, stretching dynamics of electrically tethered λ-DNA (48.5kbp) in SiO2 nanochannels has been investigated. At high electrical fields (above 20kV/m), elongations of electrically tethered DNA molecules were observed. At high E-fields, DNAs were tethered in nanochannels and were spontaneously elongated along the nanochannels up to about 90 percent of its contour length. With E-field turned off, the measured relaxation time was about 10 sec from stretching with 20kV/m. In current study, observed behaviors of DNA molecules in nanochannels were explained by field-induced dielectrophoretic DNA trap due to the particular cross-sectional geometry of nanochannels. Also the elongation ratio between 20kV/m and 60kV/m cases and the effect of E-field distribution in the transverse plane on field-induced dielectrophoretic tethering force are discussed based on “worm-like chain” model. The FEM simulation was done to verify induced dielectrophoretic tethering force into the nanohorn.
URI
http://pubs.kist.re.kr/handle/201004/38828
Appears in Collections:
KIST Publication > Conference Paper
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